Pharmaceutical Institute, Kiel University, Gutenbergstraße 76, 24118 Kiel, Germany.
Carbohydr Polym. 2017 Jun 1;165:462-469. doi: 10.1016/j.carbpol.2017.02.051. Epub 2017 Feb 20.
Fucose-containing sulfated polysaccharides (fucoidans) from brown algae exhibit anti-inflammatory activity in vivo, however, there is only limited knowledge about their mode of action. Potential targets may be the chemokine interleukin 8 (IL-8) and the anaphylatoxin C5a, as they are closely linked to inflammatory processes. In this study, two fucoidans from Saccharina latissima and Fucus vesiculosus, and unfractionated heparin (UFH) were examined for their binding properties to IL-8 and C5a and their effects on IL-8- and C5a-induced reactions of polymorphonuclear neutrophils (PMN). As proved by a competitive sulfated polysaccharide-coating-ELISA, both fucoidans bind to IL-8 and C5a, whereby they showed higher affinity to IL-8. Whereas UFH displayed only moderate effects, the fucoidans concentration-dependently reduced the IL-8- and C5a-induced intracellular calcium release, Erk1/2 phosphorylation and chemotaxis of PMN. Their inhibitory potency is dependent on the target protein, but also other aspects than the binding turned out to play a role.
褐藻中含有岩藻糖的硫酸多糖（岩藻聚糖）具有体内抗炎活性，但人们对其作用模式知之甚少。潜在的靶标可能是趋化因子白细胞介素 8（IL-8）和过敏毒素 C5a，因为它们与炎症过程密切相关。在这项研究中，我们研究了来自裙带菜和泡叶藻的两种岩藻聚糖以及未分级肝素（UFH）与 IL-8 和 C5a 的结合特性及其对 PMN 的 IL-8 和 C5a 诱导反应的影响。通过竞争性硫酸多糖包被 ELISA 证明，这两种岩藻聚糖均与 IL-8 和 C5a 结合，并且它们对 IL-8 的亲和力更高。虽然 UFH 仅显示出中等的作用，但岩藻聚糖浓度依赖性地降低了 PMN 的 IL-8 和 C5a 诱导的细胞内钙释放、Erk1/2 磷酸化和趋化性。它们的抑制效力取决于靶蛋白，但除了结合之外，其他方面也可能起作用。
