五种不同褐藻岩藻聚糖对眼部细胞氧化应激和 VEGF 干扰的影响。

Effects of Fucoidans from Five Different Brown Algae on Oxidative Stress and VEGF Interference in Ocular Cells.

机构信息

Department of Ophthalmology, University Medical Center, University of Kiel, Arnold-Heller-Str. 3, Haus 25, 24105 Kiel, Germany.

Department of Pharmaceutical Biology, Pharmaceutical Institute, University of Kiel, Gutenbergstraße 76, 24118 Kiel, Germany.

出版信息

Mar Drugs. 2019 Apr 30;17(5):258. doi: 10.3390/md17050258.

BACKGROUND

Fucoidans are interesting for potential usage in ophthalmology, and especially age-related macular degeneration. However, fucoidans from different species may vary in their effects. Here, we compare fucoidans from five algal species in terms of oxidative stress protection and vascular endothelial growth factor (VEGF) interference in ocular cells.

METHODS

Brown algae ( subsp. ) were harvested and fucoidans isolated by hot-water extraction. Fucoidans were tested in several concentrations (1, 10, 50, and 100 µg/mL). Effects were measured on a uveal melanoma cell line (OMM-1) (oxidative stress), retinal pigment epithelium (RPE) cell line ARPE19 (oxidative stress and VEGF), and primary RPE cells (VEGF). Oxidative stress was induced by HO or tert-Butyl hydroperoxide (TBHP). Cell viability was investigated with methyl thiazolyl tetrazolium (MTT or MTS) assay, and VEGF secretion with ELISA. Affinity to VEGF was determined by a competitive binding assay.

RESULTS

All fucoidans protected OMM-1 from oxidative stress. However, in ARPE19, only fucoidan from was protective. The affinity to VEGF of all fucoidans was stronger than that of heparin, and all reduced VEGF secretion in ARPE19. In primary RPE, only the fucoidan from was effective.

CONCLUSION

Among the fucoidans from five different species, displayed the most promising results concerning oxidative stress protection and reduction of VEGF secretion.

背景

褐藻胶聚糖在眼科领域具有很大的应用潜力，特别是在与年龄相关的黄斑变性方面。然而，不同物种来源的褐藻胶聚糖的作用可能有所不同。在此，我们比较了来自 5 种海藻物种的褐藻胶聚糖在眼部细胞的氧化应激保护和血管内皮生长因子（VEGF）干扰方面的作用。

方法

采集褐藻（亚种），并通过热水提取法分离褐藻胶聚糖。在几个浓度（1、10、50 和 100μg/ml）下测试褐藻胶聚糖。在葡萄膜黑色素瘤细胞系（OMM-1）（氧化应激）、视网膜色素上皮（RPE）细胞系 ARPE19（氧化应激和 VEGF）和原代 RPE 细胞（VEGF）上测量作用。通过 HO 或叔丁基过氧化物（TBHP）诱导氧化应激。通过甲基噻唑基四唑（MTT 或 MTS）测定法研究细胞活力，通过 ELISA 测定 VEGF 分泌。通过竞争性结合测定法测定与 VEGF 的亲和力。