Solairaj Dhanasekaran, Rameshthangam Palanivel, Muthukumaran Palanisamy, Wilson Jeyaraj
Department of Biotechnology, Science Campus, Alagappa University, Karaikudi 630003, Tamil Nadu, India.
Department of Biotechnology, Science Campus, Alagappa University, Karaikudi 630003, Tamil Nadu, India.
Int J Biol Macromol. 2017 Aug;101:668-679. doi: 10.1016/j.ijbiomac.2017.03.147. Epub 2017 Mar 28.
In this study, copper nanoparticle immobilized chitin nanocomposite (CNP/CuNP) was synthesized and used for the development of non-enzymatic electrochemical sensor. The CNP/CuNP was characterized by X-ray diffraction (XRD), fourier transform infra red (FTIR) spectroscopy and high resolution transmission electron microscopy (HRTEM) analysis. The glucose sensing property of CNP/CuNP was investigated by cyclic voltammetry (CV) and chronoamperometry (CA). As a result of the synergistic effect of CNP and CuNP, the modified electrode displayed effective electro-oxidation of glucose in 0.1M NaOH solution. At 0.45V potential the modified electrode showed response towards glucose in the linear range of 1-1000μM with a lowest detection limit of 0.776μM with better selectivity and stability. In addition, the antimicrobial activity of CNP/CuNP was evaluated against bacterial and fungal strains. CNP/CuNP displayed enhanced antimicrobial activity when compared to CNP and CuNP alone. Similarly, cytotoxicity of CNP/CuNP was tested against Artemia salina, which showed no toxic effect in the tested concentration.
在本研究中，合成了铜纳米颗粒固定化壳聚糖纳米复合材料（CNP/CuNP），并将其用于非酶电化学传感器的开发。通过X射线衍射（XRD）、傅里叶变换红外（FTIR）光谱和高分辨率透射电子显微镜（HRTEM）分析对CNP/CuNP进行了表征。通过循环伏安法（CV）和计时电流法（CA）研究了CNP/CuNP的葡萄糖传感性能。由于CNP和CuNP的协同作用，修饰电极在0.1M NaOH溶液中对葡萄糖表现出有效的电氧化作用。在0.45V电位下，修饰电极对葡萄糖的响应线性范围为1-1000μM，最低检测限为0.776μM，具有较好的选择性和稳定性。此外，评估了CNP/CuNP对细菌和真菌菌株的抗菌活性。与单独的CNP和CuNP相比，CNP/CuNP表现出增强的抗菌活性。同样，测试了CNP/CuNP对卤虫的细胞毒性，在测试浓度下未显示出毒性作用。
