Department of Biotechnology, Science Campus, Alagappa University, Karaikudi, 630003, Tamilnadu, India.
Department of Biotechnology, Science Campus, Alagappa University, Karaikudi, 630003, Tamilnadu, India.
Int J Biol Macromol. 2017 Dec;105(Pt 1):608-619. doi: 10.1016/j.ijbiomac.2017.07.078. Epub 2017 Jul 15.
Chitin is a natural biopolymer widely used in biomedical and environmental applications due to its distinctive physical, chemical and mechanical properties. Although the anticancer property of chitin nanoforms and chitin derivatives against various cancers were studied earlier, there is no report in the chitin nanostructure incorporated metal nanocomposite. The present study was aimed to investigate the cytotoxicity of chitin incorporated silver and copper nanocomposite against human breast cancer (MCF-7) cells. Cytotoxicity of chitin nanoparticles (CNP), silver nanoparticles (AgNP), copper nanoparticles (CuNP), chitin/silver nanocomposite (CNP/AgNP) and chitin/copper nanocomposite (CNP/CuNP) was evaluated. Among all the above, CNP/AgNP has shown a lower of 31 mg as inhibitory concentration (IC) value. Our study further showed the increased generation of reactive oxygen species with decreased activity of antioxidant enzymes and damage in the membrane integrity, thus confirms the cellular cytotoxic action of CNP/AgNP. In conclusion, the present study validates that, incorporating chitin nanoparticles with metallic nanostructure could be an effective and promising therapeutic agent against breast cancer.
壳聚糖是一种天然的生物聚合物，由于其独特的物理、化学和机械性能，广泛应用于生物医学和环境应用中。尽管壳聚糖纳米形态和壳聚糖衍生物对各种癌症的抗癌特性已被早期研究，但在壳聚糖纳米结构中加入金属纳米复合材料方面尚无报道。本研究旨在研究壳聚糖结合银和铜纳米复合材料对人乳腺癌（MCF-7）细胞的细胞毒性。评估了壳聚糖纳米颗粒（CNP）、银纳米颗粒（AgNP）、铜纳米颗粒（CuNP）、壳聚糖/银纳米复合材料（CNP/AgNP）和壳聚糖/铜纳米复合材料（CNP/CuNP）的细胞毒性。在所有这些中，CNP/AgNP 的抑制浓度（IC）值最低为 31mg。我们的研究进一步表明，活性氧的生成增加，抗氧化酶的活性降低，膜完整性受损，从而证实了 CNP/AgNP 的细胞毒性作用。总之，本研究验证了将壳聚糖纳米颗粒与金属纳米结构结合可能是一种针对乳腺癌的有效且有前途的治疗剂。
