环磷酸腺苷（cAMP）和环磷酸鸟苷（cGMP）对草履虫纤毛运动的差异调节
Differential regulation of Paramecium ciliary motility by cAMP and cGMP.
作者信息
Bonini N M, Nelson D L
机构信息
Department of Biochemistry and Neurosciences Training Program, College of Agricultural and Life Sciences, University of Wisconsin, Madison 53706.
出版信息
J Cell Biol. 1988 May;106(5):1615-23. doi: 10.1083/jcb.106.5.1615.
Abstract
cAMP and cGMP had distinct effects on the regulation of ciliary motility in Paramecium. Using detergent-permeabilized cells reactivated to swim with MgATP, we observed effects of cyclic nucleotides and interactions with Ca2+ on the swimming speed and direction of reactivated cells. Both cAMP and cGMP increased forward swimming speed two- to threefold with similar half-maximal concentrations near 0.5 microM. The two cyclic nucleotides, however, had different effects in antagonism with the Ca2+ response of backward swimming and on the handedness of the helical swimming paths of reactivated cells. These results suggest that cAMP and cGMP differentially regulate the direction of the ciliary power stroke.
摘要
环磷酸腺苷（cAMP）和环磷酸鸟苷（cGMP）对草履虫纤毛运动的调节具有不同的作用。使用经去污剂通透处理并通过MgATP重新激活以进行游动的细胞，我们观察了环核苷酸的作用以及它们与Ca2+相互作用对重新激活细胞的游动速度和方向的影响。cAMP和cGMP均使向前游动速度提高了两到三倍，其半最大浓度相似，接近0.5微摩尔。然而，这两种环核苷酸在与向后游动的Ca2+反应的拮抗作用以及对重新激活细胞螺旋游动路径的旋向性方面具有不同的作用。这些结果表明，cAMP和cGMP对纤毛动力冲程的方向具有不同的调节作用。
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