Nakaoka Y, Tanaka H, Oosawa F
J Cell Sci. 1984 Jan;65:223-31. doi: 10.1242/jcs.65.1.223.
Triton-extracted models of Paramecium cells prepared in the presence of Mg2+ and EGTA showed Ca2+ sensitivity not only in the direction of beat but also in the beat frequency of cilia. The beat frequency increased over a range of concentration of Ca2+ from 2 X 10(-7) to 4 X 10(-7) M, in which the model swam forwards. The frequency increased also above 10(-6)M-Ca2+, in which the model swam backwards. The increase in frequency was inhibited by calmodulin antagonists. Intracellular injection of a Ca2+ buffer giving a free Ca2+ concentration of 2 X 10(-7) to 5 X 10(-7) M in intact cells induced an increase in the beat frequency. Therefore, it is very likely that the beat frequency of cilia is regulated by the intracellular concentration of Ca2+.
在存在Mg2+和EGTA的情况下制备的草履虫细胞的Triton提取物模型，不仅在纤毛摆动方向上，而且在纤毛摆动频率上都表现出Ca2+敏感性。在Ca2+浓度从2×10(-7)到4×10(-7)M的范围内，模型向前游动，摆动频率增加。在Ca2+浓度高于10(-6)M时，模型向后游动，频率也增加。频率的增加受到钙调蛋白拮抗剂的抑制。在完整细胞中，细胞内注射能使游离Ca2+浓度达到2×10(-7)至5×10(-7)M的Ca2+缓冲液会导致摆动频率增加。因此，很可能纤毛的摆动频率受细胞内Ca2+浓度的调节。
