Hennessey T, Machemer H, Nelson D L
Eur J Cell Biol. 1985 Mar;36(2):153-6.
Injections of cyclic AMP (cAMP) and 8-Br-cAMP into Paramecium and external application of isobutylmethylxanthine (IBMX), an inhibitor of cAMP breakdown, to these cells increased the frequency of ciliary beating and hyperpolarized the membrane potential. When the membrane potential was held equal to the resting potential under voltage clamp, the same experimental conditions which serve to increase intracellular cAMP did not raise the ciliary frequency. We conclude that cAMP is presumably not the direct mediator of the hyperpolarization-induced ciliary activation, although it may be associated with this motor response.
向草履虫注射环磷酸腺苷（cAMP）和8-溴环磷酸腺苷（8-Br-cAMP），并将环磷酸腺苷分解抑制剂异丁基甲基黄嘌呤（IBMX）施加于这些细胞的外部，会增加纤毛摆动的频率并使膜电位超极化。当在电压钳制下将膜电位保持在静息电位时，用于增加细胞内cAMP的相同实验条件并未提高纤毛频率。我们得出结论，尽管cAMP可能与这种运动反应有关，但它可能不是超极化诱导的纤毛激活的直接介质。
