Sheibak Nadia, Heidari Zahra, Mahmoudzadeh-Sagheb Hamidreza
Department of Histology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, Iran.
Prague Med Rep. 2017;118(1):37-48. doi: 10.14712/23362936.2017.4.
Chronic periodontitis (CP), an infectious disease resulting in inflammation within the periodontal tissue, is the main cause of adult tooth loss. CP is a multi-factorial disorder and the interaction between multiple genetic and environmental factors results in the manifestation of this disease. Recent researches in periodontitis has focused on cytokine gene polymorphisms that play important role in periodontal inflammation, but few studies investigated histological change that occur during CP in the supporting tissue of teeth. The aims of this study were to investigate the association of IFN-γ +874 A/T polymorphisms and quantitative parameters of interdental gingiva in CP patients. The study samples were interdental gingiva biopsies from 60 individuals including 38 patients and 22 healthy subjects. After determination of IFN-γ +874 A/T gene polymorphism by amplification refractory mutation system-polymerase chain reaction (ARMS-PCR), patients were divided in three subgroups: 10 AA, 18 AT and 10 TT. After slides preparation, quantitative parameters were estimated by Cavalieri's point-counting method. Statistical analyses were performed using Mann-Whitney and Kruskal-Wallis test to compare differences between groups. The volume density (Vv) of epithelium, connective tissue and its components were significantly different between the control and CP groups (P<0.05). Statistically significant differences in the Vv of collagenous and non-collagenous matrix of interdental gingiva between AA, AT and TT groups were found (P<0.05). Result of present study shows that IFN-γ +874 A/T is strongly associated with some quantitative parameters of connective tissue constituents of interdental papilla in CP patients.
慢性牙周炎（CP）是一种导致牙周组织炎症的感染性疾病，是成人牙齿缺失的主要原因。CP是一种多因素疾病，多种遗传和环境因素之间的相互作用导致了这种疾病的表现。最近关于牙周炎的研究集中在细胞因子基因多态性上，这些多态性在牙周炎症中起重要作用，但很少有研究调查CP期间牙齿支持组织中发生的组织学变化。本研究的目的是调查CP患者中IFN-γ +874 A/T多态性与牙间牙龈定量参数之间的关联。研究样本是来自60个人的牙间牙龈活检组织，包括38名患者和22名健康受试者。通过扩增阻滞突变系统-聚合酶链反应（ARMS-PCR）确定IFN-γ +874 A/T基因多态性后，将患者分为三个亚组：10名AA型、18名AT型和10名TT型。制备玻片后，采用卡瓦列里点计数法估计定量参数。使用曼-惠特尼检验和克鲁斯卡尔-沃利斯检验进行统计分析，以比较组间差异。对照组和CP组之间上皮、结缔组织及其成分的体积密度（Vv）有显著差异（P<0.05）。发现AA、AT和TT组之间牙间牙龈胶原和非胶原基质的Vv有统计学显著差异（P<0.05）。本研究结果表明，IFN-γ +874 A/T与CP患者牙间乳头结缔组织成分的一些定量参数密切相关。
