运用免疫过氧化物酶试验在细胞培养中检测细胞病变型和非细胞病变型牛病毒性腹泻病毒

Detection of cytopathic and noncytopathic bovine viral diarrhea virus in cell culture with an immunoperoxidase test.

作者信息

Smith G H, Collins J K, Carman J, Minocha H C

机构信息

Diagnostic Laboratory, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80523.

出版信息

J Virol Methods. 1988 Mar-Apr;19(3-4):319-24. doi: 10.1016/0166-0934(88)90026-2.

DOI:10.1016/0166-0934(88)90026-2

PMID:2836466

Abstract

Bovine viral diarrhea virus (BVDV) antigen was detected in cell culture with an indirect immunoperoxidase (IP) procedure using a specific monoclonal antibody, and an avidin-biotin-peroxidase complex. Cytopathic and noncytopathic strains of the virus showed similar patterns of IP staining until 3 days post-infection. At six days post-infection, intensity of staining decreased in cell cultures infected with noncytopathic virus, but not with cytopathic virus. The IP procedure detected BVDV antigen in cells used to isolate virus from tissues of aborted bovine fetuses and peripheral blood lymphocytes of adult cattle. Immunoperoxidase detected BVDV isolates from 10 of 44 cases of abortion of which seven of these were noncytopathic. Noncytopathic BVDV isolates from the peripheral blood lymphocytes of 7 of 65 animals were identified.

摘要

使用特异性单克隆抗体和抗生物素蛋白-生物素-过氧化物酶复合物，通过间接免疫过氧化物酶（IP）程序在细胞培养物中检测牛病毒性腹泻病毒（BVDV）抗原。在感染后3天之前，该病毒的致细胞病变和非致细胞病变毒株显示出相似的IP染色模式。在感染后6天，感染非致细胞病变病毒的细胞培养物中染色强度降低，但感染致细胞病变病毒的细胞培养物中染色强度未降低。IP程序在用于从流产牛胎儿组织和成年牛外周血淋巴细胞中分离病毒的细胞中检测到BVDV抗原。免疫过氧化物酶在44例流产病例中的10例中检测到BVDV分离株，其中7例为非致细胞病变毒株。从65只动物中的7只的外周血淋巴细胞中鉴定出非致细胞病变BVDV分离株。