利用单克隆抗体，通过过氧化物酶-抗过氧化物酶（PAP）技术在细胞培养物和石蜡包埋组织切片中检测牛病毒性腹泻病毒抗原。
Demonstration of bovine viral diarrhoea virus antigens in cell cultures and in paraffin-embedded tissue sections by the peroxidase-antiperoxidase (PAP) technique using monoclonal antibodies.
作者信息
Belák K, Gimeno E J, Belák S
出版信息
Acta Vet Scand. 1989;30(2):231-3. doi: 10.1186/BF03548060.
Abstract
The diagnosis of both bovine viral diarrhoea (BVD) and mucosal disease (MD) is usually made on the basis of characteristic clinical and pathological findings. The definitive etiological diagnosis by virus isolation is time consuming, expensive and elusive. Isolation of the virus in cell cultures is rather difficult since it has no characteristic cytopathic effect (CPE). Furthermore, many strains have no CPE at all. Due to these uncertainties, virus isolation trials are generally supported by additional tests 1988).
摘要
牛病毒性腹泻（BVD）和黏膜病（MD）的诊断通常基于特征性的临床和病理表现。通过病毒分离进行明确的病因诊断既耗时、昂贵又难以实现。在细胞培养中分离病毒相当困难，因为它没有特征性的细胞病变效应（CPE）。此外，许多毒株根本没有CPE。由于这些不确定性，病毒分离试验通常需要其他检测的支持（1988年）。
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