Afshar A, Dulac G C, Dubuc C, Howard T H
Agriculture Canada, Animal Diseases Research Institute, Nepean, Ontario.
Can J Vet Res. 1991 Jan;55(1):91-3.
An indirect immunoperoxidase staining technique (IP) is described for the detection of bovine viral diarrhea virus (BVDV) in bovine semen. The performance of the IP was compared to the reference immunofluorescent staining test in its ability to detect BVDV in 23 coded field semen samples. The IP assay which can be applied with ease to a large number of samples and does not require expensive fluorescence microscope equipment, appears to be an alternative method for BVDV detection. The IP assay can be strongly recommended for certification of BVDV-free bovine semen for artificial insemination and trading purposes and for laboratories which are not equipped for performing the immunofluorescent test.
本文描述了一种间接免疫过氧化物酶染色技术（IP），用于检测牛精液中的牛病毒性腹泻病毒（BVDV）。将IP的性能与其在检测23份编码的现场精液样本中BVDV的能力方面的参考免疫荧光染色试验进行了比较。IP检测可以轻松应用于大量样本，并且不需要昂贵的荧光显微镜设备，似乎是检测BVDV的一种替代方法。对于用于人工授精和交易目的的无BVDV牛精液的认证以及对于没有配备进行免疫荧光试验设备的实验室，强烈推荐使用IP检测。
