通过灵敏且可靠的酶联免疫吸附测定法（ELISA）以绝对单位测量杂交瘤上清液和腹水中小鼠IgG单克隆抗体的浓度。
Measurement of the concentration of murine IgG monoclonal antibody in hybridoma supernatants and ascites in absolute units by sensitive and reliable enzyme-linked immunosorbent assays (ELISA).
作者信息
Fleming J O, Pen L B
机构信息
Department of Neurology, USC School of Medicine, Los Angeles 90033.
出版信息
J Immunol Methods. 1988 May 25;110(1):11-8. doi: 10.1016/0022-1759(88)90077-4.
Abstract
We have investigated an enzyme-linked immunosorbent assay (ELISA) for mouse IgG using affinity-purified goat anti-mouse antibodies for capture and detection. This assay was used to measure the absolute or weight/volume concentration of murine monoclonal antibody in hybridoma supernatants. Bovine or subclasses except IgG3 in the 1-20 ng/ml range. Antibody capture was essentially complete in the optimized assay. In combination with an antigen-dependent ELISA, the assay allowed estimation of the absolute concentration of specific monoclonal antibody in ascites. These rapid and relatively simple assays may be applicable in many situations in which a practical means of measuring murine monoclonal antibodies in weight/volume units is needed.
摘要
我们研究了一种用于检测小鼠IgG的酶联免疫吸附测定（ELISA）方法，该方法使用亲和纯化的山羊抗小鼠抗体进行捕获和检测。此测定法用于测量杂交瘤上清液中鼠单克隆抗体的绝对浓度或重量/体积浓度。在1 - 20 ng/ml范围内，除IgG3外的牛血清或亚类。在优化的测定中，抗体捕获基本完成。结合抗原依赖性ELISA，该测定法可估算腹水中特异性单克隆抗体的绝对浓度。这些快速且相对简单的测定法可能适用于许多需要以重量/体积单位实际测量鼠单克隆抗体的情况。
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