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An enzyme-linked immunosorbent assay for the quantitation of human IgG subclasses using monoclonal antibodies.

作者信息

Ferrante A, Rowan-Kelly B, Beard L J, Maxwell G M

出版信息

J Immunol Methods. 1986 Nov 6;93(2):207-12. doi: 10.1016/0022-1759(86)90190-0.

DOI:10.1016/0022-1759(86)90190-0
PMID:3772114
Abstract

An enzyme-linked immunosorbent assay was established for the quantitation of human IgG1, IgG2, IgG3 and IgG4 using IgG subclass-specific monoclonal antibodies. The method could detect 1-10 ng/ml of the Ig subclasses. The technique is suitable for measuring IgG subclass concentration in sera of healthy adults and in supernatants from human lymphocytes cultured in the presence of pokeweed mitogen.

摘要

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