Instituto de Tecnologia Química e Biológica António Xavier/Universidade Nova de Lisboa (ITQB NOVA), Av. da Republica-EAN, 2780-157 Oeiras, Portugal.
Instituto de Tecnologia Química e Biológica António Xavier/Universidade Nova de Lisboa (ITQB NOVA), Av. da Republica-EAN, 2780-157 Oeiras, Portugal.
Bioresour Technol. 2017 Jul;235:149-156. doi: 10.1016/j.biortech.2017.03.091. Epub 2017 Mar 20.
In this work a novel bioprocess for hydrogenation of CO to formate was developed, using whole cell catalysis by a sulfate-reducing bacterium. Three Desulfovibrio species were tested (D. vulgaris Hildenborough, D. alaskensis G20, and D. desulfuricans ATCC 27774), of which D. desulfuricans showed the highest activity, producing 12mM of formate in batch, with a production rate of 0.09mMh. Gene expression analysis indicated that among the three formate dehydrogenases and five hydrogenases, the cytoplasmic FdhAB and the periplasmic [FeFe] HydAB are the main enzymes expressed in D. desulfuricans in these conditions. The new bioprocess for continuous formate production by D. desulfuricans had a maximum specific formate production rate of 14mMgh, and more than 45mM of formate were obtained with a production rate of 0.40mMh. This is the first report of a continuous process for biocatalytic formate production.
在这项工作中，开发了一种新型的生物过程，使用硫酸盐还原菌进行全细胞催化，将 CO 氢化生成甲酸盐。测试了三种脱硫弧菌（D. vulgaris Hildenborough、D. alaskensis G20 和 D. desulfuricans ATCC 27774），其中 D. desulfuricans 表现出最高的活性，在分批培养中产生 12mM 的甲酸盐，生产速率为 0.09mMh。基因表达分析表明，在三种甲酸盐脱氢酶和五种氢化酶中，细胞质 FdhAB 和周质 [FeFe] HydAB 是 D. desulfuricans 在这些条件下主要表达的酶。D. desulfuricans 连续生产甲酸盐的新生物过程的最大比甲酸盐生产速率为 14mMgh，获得了超过 45mM 的甲酸盐，生产速率为 0.40mMh。这是连续生物催化甲酸盐生产过程的首次报道。
