Bulat Zorica, Đukić-Ćosić Danijela, Antonijević Biljana, Buha Aleksandra, Bulat Petar, Pavlović Zoran, Matović Vesna
Department of Toxicology "Akademik Danilo Soldatović", Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11221 Belgrade.
Department of Toxicology "Akademik Danilo Soldatović", Faculty of Pharmacy.
Arh Hig Rada Toksikol. 2017 Mar 1;68(1):38-45. doi: 10.1515/aiht-2017-68-2919.
The study was designed to investigate the influence of zinc (Zn) supplementation on cadmium-induced alterations in zinc, copper (Cu), and magnesium (Mg) status in rabbits. For this purpose, the concentrations of cadmium (Cd), Zn, Cu, and Mg were estimated in the blood, liver, kidney, and bone. The rabbits were divided in a control group, a Cd group-animals intoxicated orally with Cd (10 mg kg-1 bw, as aqueous solution of Cd-chloride), and a Cd+Zn group-animals intoxicated with the same dose of Cd and co-treated with Zn (20 mg kg-1 bw, as aqueous solution of Zn-sulphate). Solutions were administered orally, every day for 28 days. Sample mineralisation was performed with concentrated nitric acid (HNO3) and perchloric acid (HClO4) (4:1) and metal concentrations were determined by atomic absorption spectrophotometry (AAS). Zinc supplementation improved some of Cd-induced disturbances in bioelement levels in the investigated tissues. Beneficial effects of Zn on Zn and Cu levels were observed in blood, as well as on the Cu kidney level. The calculated values for Cu/Zn, Mg/Zn, and Mg/Cu ratios in blood suggest that Zn co-treatment reduces Cd-induced changes in bioelement ratios in blood.
本研究旨在探讨补充锌（Zn）对镉诱导的家兔锌、铜（Cu）和镁（Mg）状态改变的影响。为此，对血液、肝脏、肾脏和骨骼中的镉（Cd）、锌、铜和镁浓度进行了测定。将家兔分为对照组、镉组（动物经口摄入镉（10 mg kg-1体重，以氯化镉水溶液形式））和镉+锌组（动物摄入相同剂量的镉并同时接受锌处理（20 mg kg-1体重，以硫酸锌水溶液形式））。溶液每天经口给药，持续28天。样品用浓硝酸（HNO3）和高氯酸（HClO4）（4:1）进行矿化处理，金属浓度通过原子吸收分光光度法（AAS）测定。补充锌改善了所研究组织中一些镉诱导的生物元素水平紊乱。在血液中以及肾脏铜水平上观察到锌对锌和铜水平的有益影响。血液中铜/锌、镁/锌和镁/铜比值的计算值表明，锌联合处理减少了镉诱导的血液中生物元素比值变化。
