University of Belgrade Faculty of Pharmacy, Department of Toxicology "Akademik Danilo Soldatović", Belgrade, Serbia.
Health Centre Kosovska Mitrovica, Kosovska Mitrovica, Serbia.
Arh Hig Rada Toksikol. 2020 Oct 6;71(3):197-204. doi: 10.2478/aiht-2020-71-3425. Print 2020 Sep 1.
Most Pb and Cd neurotoxicity studies investigate exposure to either of the toxic metals alone, while data on co-exposure are scarce. The aim of our study was to fill that gap by investigating acute combined effects of Pb and Cd on redox and essential metal status in the brain of Wistar rats. Animals were randomised in four groups of six to eight rats, which received 15 or 30 mg/kg of Cd, 150 mg/kg of Pb, or 150 mg/kg of Pb + 15 mg/kg of Cd by gavage. The fifth, control, group received distilled water only. Co-treatment with Pb and Cd induced significant increase in malondialdehyde (MDA) and thiobarbituric acid-reactive substances (TBARS) compared to control and groups receiving either metal alone. This is of special importance, as MDA presence in the brain has been implicated in many neurodegenerative disorders. The groups did not significantly differ in Zn, Cu, Mn, and Fe brain levels. Our findings highlight the importance of metal mixture studies. Neurotoxicity assessments of single chemicals do not provide a real insight into exposure to mixtures in real life. Further research should look into interactions between these metals to reveal complex molecular mechanisms of their neurotoxicity.
大多数关于铅（Pb）和镉（Cd）神经毒性的研究都只调查了单一有毒金属的暴露情况，而关于联合暴露的数据则很少。我们的研究旨在通过研究 Pb 和 Cd 对 Wistar 大鼠大脑的氧化还原和必需金属状态的急性联合效应来填补这一空白。动物被随机分为四组，每组六到八只大鼠，分别通过灌胃给予 15 或 30mg/kg 的 Cd、150mg/kg 的 Pb 或 150mg/kg 的 Pb+15mg/kg 的 Cd。第五组，即对照组，只接受蒸馏水。与对照组和单独接受任一金属的组相比，Pb 和 Cd 的联合处理诱导了丙二醛（MDA）和硫代巴比妥酸反应物质（TBARS）的显著增加。这一点尤为重要，因为 MDA 在大脑中的存在与许多神经退行性疾病有关。各组的锌（Zn）、铜（Cu）、锰（Mn）和铁（Fe）在大脑中的含量没有显著差异。我们的研究结果强调了金属混合物研究的重要性。对单一化学物质的神经毒性评估不能真正深入了解现实生活中对混合物的暴露情况。进一步的研究应该研究这些金属之间的相互作用，以揭示它们神经毒性的复杂分子机制。
