Postic Guillaume, Ghouzam Yassine, Etchebest Catherine, Gelly Jean-Christophe
Inserm U1134, Paris, France.
Université Paris Diderot, Sorbonne Paris Cité, UMR_S 1134, Paris, France.
Database (Oxford). 2017 Jan 1;2017(1). doi: 10.1093/database/bax022.
Knowing the position of protein structures within the membrane is crucial for fundamental and applied research in the field of molecular biology. Only few web resources propose coordinate files of oriented transmembrane proteins, and these exclude predicted structures, although they represent the largest part of the available models. In this article, we present TMPL (http://www.dsimb.inserm.fr/TMPL/), a database of transmembrane protein structures (α-helical and β-sheet) positioned in the lipid bilayer. It is the first database to include theoretical models of transmembrane protein structures, making it a large repository with more than 11 000 entries. The TMPL database also contains experimentally solved protein structures, which are available as either atomistic or coarse-grained models. A unique feature of TMPL is the possibility for users to update the database by uploading, through an intuitive web interface, the membrane assignments they can obtain with our recent OREMPRO web server.
了解蛋白质结构在膜内的位置对于分子生物学领域的基础研究和应用研究至关重要。只有少数网络资源提供定向跨膜蛋白的坐标文件，而且这些资源不包括预测结构，尽管预测结构占现有模型的最大部分。在本文中，我们介绍了TMPL（http://www.dsimb.inserm.fr/TMPL/），这是一个位于脂质双层中的跨膜蛋白结构（α螺旋和β折叠）数据库。它是第一个包含跨膜蛋白结构理论模型的数据库，使其成为一个拥有超过11000个条目的大型存储库。TMPL数据库还包含通过实验解析的蛋白质结构，这些结构可以作为原子模型或粗粒度模型获取。TMPL的一个独特功能是用户可以通过直观的网络界面上传他们使用我们最近的OREMPRO网络服务器获得的膜分配结果来更新数据库。
