Abraham G, Colonno R J
Department of Virus and Cell Biology, Merck Sharp and Dohme Research Laboratories, West Point, Pennsylvania 19486.
J Virol. 1988 Jul;62(7):2300-6. doi: 10.1128/JVI.62.7.2300-2306.1988.
The attachment of rhinoviruses to cellular receptors was studied by displacing bound virus particles with an anti-receptor monoclonal antibody. The two serotypes studied differed significantly with respect to the temperature dependence of displacement and the nature of the particles displaced. Binding was shown to be a two-step process, the first of which is reversible and is seen when viruses are bound either to isolated cell membranes or to cells at lower than physiological temperatures. Second-stage binding was seen with serotype 14 when bound to intact cells. Viral particles released from such cells by incubation at 37 degrees C or by anti-receptor antibody exhibited altered physical changes in the capsid and a loss of infectivity. In contrast, serotype 67 bound efficiently to cells at 37 degrees C and did not elute spontaneously but could be displaced by anti-receptor antibody to produce complete, infectious particles. Rhinoviruses labeled with [3H]myristic acid or with [35S]methionine were displaced similarly from cells or membranes by anti-receptor antibody, indicating that the majority of VP4 of rhinoviruses does not enter or remain attached to cells during either the first or second stage of virus binding. These data support the conclusion that the myristic acid moiety of VP4 is not involved in the initial viral interaction with cellular receptors.
通过用抗受体单克隆抗体取代结合的病毒颗粒，研究了鼻病毒与细胞受体的附着情况。所研究的两种血清型在取代的温度依赖性和被取代颗粒的性质方面存在显著差异。结合显示为一个两步过程，第一步是可逆的，当病毒在低于生理温度下与分离的细胞膜或细胞结合时可见。血清型14与完整细胞结合时可见第二阶段结合。通过在37℃孵育或用抗受体抗体从这些细胞中释放的病毒颗粒在衣壳中表现出物理变化改变和感染性丧失。相比之下，血清型67在37℃时能有效地与细胞结合，不会自发洗脱，但可被抗受体抗体取代以产生完整的感染性颗粒。用[3H]肉豆蔻酸或[35S]甲硫氨酸标记的鼻病毒被抗受体抗体从细胞或膜上类似地取代，这表明在病毒结合的第一阶段或第二阶段，鼻病毒的大多数VP4不会进入细胞或保持附着在细胞上。这些数据支持了VP4的肉豆蔻酸部分不参与病毒与细胞受体初始相互作用的结论。
