Department of Oral Medicine, Infection, and Immunity, Harvard School of Dental Medicine, Boston, MA 02115, USA.
Department of Oral Medicine, Infection, and Immunity, Harvard School of Dental Medicine, Boston, MA 02115, USA; Advanced Microscopy Program, Center for Systems Biology and Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.
Stem Cell Reports. 2017 Apr 11;8(4):933-946. doi: 10.1016/j.stemcr.2017.03.002. Epub 2017 Mar 30.
Post-natal skeletal stem cells expressing PRX1 (pnPRX1+) have been identified in the calvaria and in the axial skeleton. Here we characterize the location and functional capacity of the calvarial pnPRX1 cells. We found that pnPRX1 reside exclusively in the calvarial suture niche and decrease in number with age. They are distinct from preosteoblasts and osteoblasts of the sutures, respond to WNT signaling in vitro and in vivo by differentiating into osteoblasts, and, upon heterotopic transplantation, are able to regenerate bone. Diphtheria toxin A (DTA)-mediated lineage ablation of pnPRX1 cells and suturectomy perturb regeneration of calvarial bone defects and confirm that pnPRX1 cells of the sutures are required for bone regeneration. Orthotopic transplantation of sutures with traceable pnPRX1 cells into wild-type animals shows that pnPRX1 cells of the suture contribute to calvarial bone defect regeneration. DTA-mediated lineage ablation of pnPRX1 does not, however, interfere with calvarial development.
在颅骨和轴骨中已经鉴定出表达 PRX1（pnPRX1+）的产后骨骼干细胞。在这里，我们描述了颅骨 pnPRX1 细胞的位置和功能能力。我们发现 pnPRX1 仅存在于颅骨缝的龛位中，并随着年龄的增长而减少。它们与骨缝中的前成骨细胞和成骨细胞不同，在体外和体内对 WNT 信号有反应，分化为成骨细胞，并且在异位移植后能够再生骨骼。白喉毒素 A（DTA）介导的 pnPRX1 细胞谱系消融和骨缝切除术扰乱了颅骨骨缺损的再生，并证实骨缝中的 pnPRX1 细胞是骨再生所必需的。将可追踪的 pnPRX1 细胞的骨缝进行同源移植到野生型动物中，表明骨缝中的 pnPRX1 细胞有助于颅骨骨缺损的再生。然而，DTA 介导的 pnPRX1 细胞谱系消融并不干扰颅骨的发育。
