Brucksch A, Gröne H J, Talartschik J, Fuchs E
Deutsches Primatenzentrum, Göttingen.
Klin Wochenschr. 1988 Apr 1;66(7):303-7. doi: 10.1007/BF01727517.
Specific binding sites for atrial natriuretic peptide (99-126) in different areas of normal human renal tissue were quantified by in vitro autoradiography. Our data represent the first characterization of ANP binding sites in different structures of the human kidney. Characterization of ANP binding revealed by Scatchard plot analysis a single class of high affinity binding sites in the glomeruli (Kd 0.53 +/- 0.11 nM; BMax 74.4 +/- 17.86 fmol/mg protein), the vasculature (Kd 0.18 +/- 0.014 nM; BMax 91.6 +/- 25.02 fmol/mg protein), and the medulla (Kd 0.34 +/- 0.13 nM; BMax 106.0 +/- 30.61 fmol/mg protein). These sites may play a key role in the actions of the cardiac hormone in human kidney and in the ameliorating effects of ANP in the recovery from acute renal failure.
通过体外放射自显影法对正常人肾组织不同区域中心房利钠肽（99 - 126）的特异性结合位点进行了定量分析。我们的数据首次描述了人肾不同结构中的心钠素结合位点。通过Scatchard图分析揭示的心钠素结合特征显示，在肾小球（解离常数Kd为0.53±0.11 nM；最大结合容量BMax为74.4±17.86 fmol/mg蛋白）、脉管系统（Kd为0.18±0.014 nM；BMax为91.6±25.02 fmol/mg蛋白）和髓质（Kd为0.34±0.13 nM；BMax为106.0±30.61 fmol/mg蛋白）中存在单一类别的高亲和力结合位点。这些位点可能在心脏激素对人肾的作用以及心钠素对急性肾衰竭恢复的改善作用中发挥关键作用。
