Herrmann A, Schulz W, Hahlbrock K
Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Köln, Federal Republic of Germany.
Mol Gen Genet. 1988 Apr;212(1):93-8. doi: 10.1007/BF00322449.
Two types of genomic DNA hybridizing with a chalcone synthase cDNA were isolated from cell suspension cultures of parsley (Petroselinum crispum cv. Mooskrause) and cloned in lambda EMBL4. Their fragmentation patterns with several common restriction enzymes were identical, except for the occurrence of a 927 base pair insertion in one type relative to the other. This insertion is located 538 base pairs upstream of the first of two transcription start sites and has characteristic features of a transposable element. The two types of cloned DNA most likely represent two alleles of a chalcone synthase gene occurring in one copy per haploid parsley genome. The nucleotide sequence and exon-intron structure of the larger allele were determined. Analysis of plants either heterozygous or homozygous with respect to the chalcone synthase gene revealed that both allelic forms were expressed and activated by UV light.
从欧芹（皱叶欧芹品种Mooskrause）的细胞悬浮培养物中分离出两种与查尔酮合酶cDNA杂交的基因组DNA，并克隆到λEMBL4中。它们用几种常见限制酶的片段化模式相同，只是其中一种类型相对于另一种类型有一个927个碱基对的插入。该插入位于两个转录起始位点中第一个的上游538个碱基对处，具有转座元件的特征。这两种克隆的DNA很可能代表单倍体欧芹基因组中每个拷贝出现一次的查尔酮合酶基因的两个等位基因。测定了较大等位基因的核苷酸序列和外显子-内含子结构。对查尔酮合酶基因杂合或纯合的植物分析表明，两种等位基因形式均表达并受紫外线激活。
