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本文引用的文献

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UV-induction of chalcone synthase mRNA in cell suspension cultures of Petroselinum hortense.甜茴香细胞悬浮培养物中查尔酮合酶 mRNA 的 UV 诱导。
Proc Natl Acad Sci U S A. 1983 May;80(9):2591-3. doi: 10.1073/pnas.80.9.2591.
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In situ localization of light-induced chalcone synthase mRNA, chalcone synthase, and flavonoid end products in epidermal cells of parsley leaves.在欧芹叶片表皮细胞中光诱导的查尔酮合酶 mRNA、查尔酮合酶和类黄酮终产物的原位定位。
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UV-inducible transient expression in parsley protoplasts identifies regulatory cis-elements of a chimeric Antirrhinum majus chalcone synthase gene.紫外线诱导的欧芹原生质体中的瞬时表达鉴定了杂种金鱼草查尔酮合酶基因的调控顺式元件。
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Highly purified "flavanone synthase" from parsley catalyzes the formation of naringenin chalcone.从欧芹中高度纯化得到的“黄烷酮合酶”催化柚皮素查耳酮的形成。
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A plant flavone, luteolin, induces expression of Rhizobium meliloti nodulation genes.一种植物黄酮,木犀草素,可诱导苜蓿根瘤菌结瘤基因的表达。
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Detection of factors that interact with the human beta-interferon regulatory region in vivo by DNAase I footprinting.通过DNA酶I足迹法在体内检测与人β-干扰素调控区相互作用的因子。
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来自欧芹的光响应查尔酮合酶启动子的功能结构

Functional architecture of the light-responsive chalcone synthase promoter from parsley.

作者信息

Schulze-Lefert P, Becker-André M, Schulz W, Hahlbrock K, Dangl J L

机构信息

Department of Biochemistry, Max-Planck-Institut für Züchtungsforschung, Köln, Federal Republic of Germany.

出版信息

Plant Cell. 1989 Jul;1(7):707-14. doi: 10.1105/tpc.1.7.707.

DOI:10.1105/tpc.1.7.707
PMID:2535519
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC159807/
Abstract

We have combined in vivo genomic footprinting and light-induced transient expression of chalcone synthase promoter derivatives in parsley protoplasts to identify cis sequences regulating light activation. The parsley chalcone synthase promoter contains two cis "units" that are light-responsive. Each unit is composed of short DNA stretches of approximately 50 base pairs, and each contains two in vivo footprints. One of the footprints in each unit covers a sequence that is highly conserved among other light- and stress-regulated plant genes. The other footprinted sequences in each unit are not related to each other. The TATA distal light-responsive unit is inherently weak but can compensate partially for the loss of the stronger TATA proximal unit. Levels of light-induced expression from either can be influenced by the presence of a region of approximately 100 base pairs located upstream of the TATA distal light-responsive unit. Combination of the light-responsive units and upstream region generates a synergistic response to light. We speculate that functional compensation generated by nonidentical, but sequence-related, cis units foreshadows combinatorial diversity of cognate trans factors.

摘要

我们将体内基因组足迹分析与查尔酮合酶启动子衍生物在欧芹原生质体中的光诱导瞬时表达相结合,以鉴定调控光激活的顺式作用序列。欧芹查尔酮合酶启动子包含两个对光有响应的顺式“单元”。每个单元由大约50个碱基对的短DNA片段组成,且每个单元都有两个体内足迹。每个单元中的一个足迹覆盖了在其他光和胁迫调控的植物基因中高度保守的序列。每个单元中其他有足迹的序列彼此不相关。TATA远端光响应单元本身较弱,但可以部分补偿较强的TATA近端单元的缺失。任一单元的光诱导表达水平都可能受到位于TATA远端光响应单元上游约100个碱基对区域的影响。光响应单元与上游区域的组合对光产生协同反应。我们推测,由不相同但序列相关的顺式单元产生的功能补偿预示着同源反式作用因子的组合多样性。