糖网一方对糖尿病大鼠视网膜血管的保护作用

Protective Effect of Tang Wang One Decoction on the Retinal Vessels of Diabetic Rats.

作者信息

Kou Xinyun, Yang Shufei, Qin Yali, Yang Chao, Deng Tingting, Luo Dan, Jin Ming

机构信息

China-Japan Friendship Hospital, No. 2, Yinghua East Street, Chaoyang District, Beijing 100029, China; Xicheng Maternal and Child Health Hospital, No. 19, Pingyuan Street, Xicheng District, Beijing 100054, China.

China-Japan Friendship Hospital, No. 2, Yinghua East Street, Chaoyang District, Beijing 100029, China.

出版信息

Evid Based Complement Alternat Med. 2017;2017:8635127. doi: 10.1155/2017/8635127. Epub 2017 Mar 6.

. This study aimed to determine the influence of Tang Wang One Decoction (TWOD) on the retinal vessels of diabetic rats. . The hemorheology of diabetic rats was observed. Morphological studies of retinal vessels were conducted using optical microscopy and electron microscopy. Immunological histochemistry assay was used to measure the expression levels of MMP-9, occludin, and claudin-5. . Obvious pathological damage was observed in the retinal vessels of diabetic rats. TWOD positively affected the hemorheology and morphology of retinal vessels. The decoction also decreased the expression of MMP-9 and increased the expression of occludin and claudin-5. . The results suggest that the retinal protective effects of TWOD might be related to downregulation of MMP-9 and upregulation of occludin and claudin-5.

本研究旨在确定糖网一方汤（TWOD）对糖尿病大鼠视网膜血管的影响。观察糖尿病大鼠的血液流变学。使用光学显微镜和电子显微镜对视网膜血管进行形态学研究。采用免疫组织化学分析法检测基质金属蛋白酶-9（MMP-9）、闭合蛋白和紧密连接蛋白-5的表达水平。糖尿病大鼠的视网膜血管出现明显的病理损伤。糖网一方汤对视网膜血管的血液流变学和形态学有积极影响。该汤剂还降低了MMP-9的表达，增加了闭合蛋白和紧密连接蛋白-5的表达。结果表明，糖网一方汤的视网膜保护作用可能与下调MMP-9以及上调闭合蛋白和紧密连接蛋白-5有关。