Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisboa 1649-004, Portugal.
Department of Medicine and Ophthalmology, Boston University School of Medicine, Boston, MA 02118, USA.
Biomolecules. 2020 Nov 21;10(11):1583. doi: 10.3390/biom10111583.
Changes in retinal microcirculation are associated with the development of diabetic retinopathy (DR). However, it is unclear whether such changes also develop in capillary beds of other non-retinal tissues. Here, we investigated microcirculatory changes involving velocity of rolling neutrophils, adherence of neutrophils, and leukostasis during development of retinal vascular lesions in diabetes in other non-retinal tissues. Intravital microscopy was performed on post-capillary venules of cremaster muscle and ear lobe of mice with severe or moderate diabetes and compared to those of non-diabetic mice. Additionally, number and velocity of rolling leukocytes, number of adherent leukocytes, and areas of leukostasis were quantified, and retinal capillary networks were examined for acellular capillaries (AC) and pericyte loss (PL), two prominent vascular lesions characteristic of DR. The number of adherent neutrophils and areas of leukostasis in the cremaster and ear lobe post-capillary venules of diabetic mice was increased compared to those of non-diabetic mice. Similarly, a significant increase in the number of rolling neutrophils and decrease in their rolling velocities compared to those of non-diabetic control mice were observed and severity of diabetes exacerbated these changes. Understanding diabetes-induced microcirculatory changes in cremaster and ear lobe may provide insight into retinal vascular lesion development in DR.
视网膜微循环的变化与糖尿病性视网膜病变 (DR) 的发展有关。然而，目前尚不清楚这种变化是否也会发生在其他非视网膜组织的毛细血管床中。在这里，我们研究了在糖尿病视网膜血管病变发展过程中，涉及滚动中性粒细胞速度、中性粒细胞黏附以及白细胞淤滞的其他非视网膜组织中的微循环变化。对严重或中度糖尿病小鼠的肠系膜后小静脉和耳垂毛细血管后小静脉进行活体显微镜检查，并与非糖尿病小鼠进行比较。此外，还定量了滚动白细胞的数量和速度、黏附白细胞的数量以及白细胞淤滞的面积，并检查了视网膜毛细血管网络中无细胞毛细血管 (AC) 和周细胞丢失 (PL) 的情况，这是 DR 的两种突出的血管病变。与非糖尿病小鼠相比，糖尿病小鼠肠系膜和耳垂毛细血管后小静脉中的黏附中性粒细胞数量和白细胞淤滞面积增加。同样，与非糖尿病对照小鼠相比，滚动中性粒细胞的数量显著增加，滚动速度降低，并且糖尿病的严重程度加剧了这些变化。了解肠系膜和耳垂中糖尿病引起的微循环变化可能有助于深入了解 DR 中视网膜血管病变的发展。
