恶性疟原虫两个小亚基核糖体RNA基因的一级序列。

Primary sequences of two small subunit ribosomal RNA genes from Plasmodium falciparum.

作者信息

McCutchan T F, de la Cruz V F, Lal A A, Gunderson J H, Elwood H J, Sogin M L

机构信息

Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD.

出版信息

Mol Biochem Parasitol. 1988 Feb;28(1):63-8. doi: 10.1016/0166-6851(88)90181-8.

DOI:10.1016/0166-6851(88)90181-8

PMID:2836731

Abstract

We have determined the complete sequence of two structurally distinct 18S ribosomal RNA genes from the malarial parasite Plasmodium falciparum. S1 nuclease analyses demonstrate that only one of the genes is represented in stable rRNA populations isolated from blood-stage parasites. Comparisons of homologous rRNA genes from Plasmodium berghei and P. falciparum reveal that they are identical at 86% of their positions. From comparisons of the Plasmodium genes to that of humans, it was possible to design genus-specific as well as species-specific oligonucleotide probes that can be used to distinguish the parasite 18S ribosomal RNA from that of its host. The utilization of these probes as diagnostic reagents is discussed.

摘要

我们已经确定了来自疟原虫恶性疟原虫的两个结构不同的18S核糖体RNA基因的完整序列。S1核酸酶分析表明，从血液阶段寄生虫中分离出的稳定rRNA群体中仅代表其中一个基因。来自伯氏疟原虫和恶性疟原虫的同源rRNA基因的比较显示，它们在86%的位置上是相同的。通过将疟原虫基因与人类基因进行比较，有可能设计出属特异性和种特异性的寡核苷酸探针，可用于区分寄生虫18S核糖体RNA与其宿主的18S核糖体RNA。讨论了将这些探针用作诊断试剂的用途。