Gad Ahmed H
Department of Biological Science, University of Tulsa, 800 South Tucker Drive, Oliphant Hall 312, Tulsa, OK 74104, United States.
Genom Data. 2017 Mar 8;12:54-57. doi: 10.1016/j.gdata.2017.03.002. eCollection 2017 Jun.
The Great Salt Plains of Oklahoma (GSP) is an extreme region, a hypersaline environment from marine origin and a unique area of the Salt National Wild Refuge in the north-central region of Oklahoma. In this study we analyzed the diversity and distribution of bacteria in two habitats; vegetated areas (GAB) and salt flat areas (GAS) in the sediments of GSP using the high-throughput techniques of 16S rRNA gene amplicon (V1-V2 regions) metagenomics-454 pyrosequencing. The filtered sequences resulted to a total of 303,723 paired end reads were generated, assigned into 1646 numbers of OTUs and 56.4% G + C content for GAB, and a total of 144,496 paired end reads were generated, assigned into 785 numbers of OTUs and 56.7% G + C content for GAS. All the resulting 16S rRNA was of an average length ~ 187 bp, assigned to 37 bacterial phyla and candidate divisions. The abundant OTUs were affiliated with Proteobacteria (36.2% in GAB and 31.5% in GAS), Alphaproteobacteria (13.3% in GAB and 8.7% in GAS), Gammaproteobacteria (13% in GAB and 14.2% in GAS), Deltaproteobacteria (6.5% in GAB and 6.1% in GAS), Betaproteobacteria (2.6% in GAB and 1.14% in GAS), Bacteroidetes (16.8% in GAB and 24.3% in GAS), Chloroflexi (8.7% in GAB and 6% in GAS), Actinobacteria (8.5% in GAB and 5.8% in GAS) and Firmicutes (6.5% in GAB and 6.6% in GAS). This is the first study of a high resolution microbial phylogenetic profile of the GSP and the findings stipulate evidence of the bacterial heterogeneity that might be originated by surface and subsurface environments and better understanding of the ecosystem dynamics of GSP. Metagenome sequence data are available at NCBI with accession numbers; LT699840-LT700186.
俄克拉荷马大盐原（GSP）是一个极端区域，是源自海洋的高盐环境，也是俄克拉荷马州中北部盐类国家野生动物保护区的一个独特区域。在本研究中，我们使用16S rRNA基因扩增子（V1 - V2区域）宏基因组学 - 454焦磷酸测序的高通量技术，分析了GSP沉积物中两个栖息地——植被覆盖区域（GAB）和盐滩区域（GAS）的细菌多样性和分布情况。经筛选后，GAB共产生了303,723条双端读数，归为1646个操作分类单元（OTU），G + C含量为56.4%；GAS共产生了144,496条双端读数，归为785个OTU，G + C含量为56.7%。所有得到的16S rRNA平均长度约为187 bp，归属于37个细菌门和候选类群。优势OTU隶属于变形菌门（GAB中占36.2%，GAS中占31.5%）、α-变形菌纲（GAB中占13.3%，GAS中占8.7%）、γ-变形菌纲（GAB中占13%，GAS中占14.2%）、δ-变形菌纲（GAB中占6.5%，GAS中占6.1%）、β-变形菌纲（GAB中占2.6%，GAS中占1.14%）、拟杆菌门（GAB中占16.8%，GAS中占24.3%）、绿弯菌门（GAB中占8.7%，GAS中占6%）、放线菌门（GAB中占8.5%，GAS中占5.8%）和厚壁菌门（GAB中占6.5%，GAS中占6.6%）。这是首次对GSP进行高分辨率微生物系统发育图谱研究，研究结果为可能由地表和地下环境导致的细菌异质性提供了证据，并有助于更好地理解GSP的生态系统动态。宏基因组序列数据可在NCBI获取，登录号为：LT699840 - LT700186。
