Altanerová V, Altaner C
Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
Neoplasma. 1988;35(2):207-13.
Hamster cells transformed with avian sarcoma virus, which had been selected for resistance to 8-azaguanine, were cloned. Several single cell clones were isolated which differed significantly in their tumorigenicity in comparison to the parental highly tumorigenic cells. The nontumorigenic cell clones contained rescuable avian virus. Both parental and resistant cells possessed transformed phenotype. Comparative studies with parental highly tumorigenic cells and nontumorigenic cell clones failed to detect any difference in the expression of p60src, in this phosphorylation and in phosphokinase activity. The isolated nontumorigenic cells represent cell mutants in which the viral src gene is controlled by a suppressor gene. The cells might be useful for further characterization of a putative antioncogene.
用禽肉瘤病毒转化并经筛选获得对8-氮鸟嘌呤耐药性的仓鼠细胞被克隆。分离出了几个单细胞克隆，与亲代高致瘤性细胞相比，它们的致瘤性有显著差异。非致瘤性细胞克隆含有可拯救的禽病毒。亲代细胞和耐药细胞均具有转化表型。对亲代高致瘤性细胞和非致瘤性细胞克隆进行的比较研究未能检测到p60src在表达、磷酸化及磷酸激酶活性方面存在任何差异。分离出的非致瘤性细胞代表了细胞突变体，其中病毒src基因受一个抑制基因控制。这些细胞可能有助于进一步鉴定一个假定的抗癌基因。
