Ahmed Sibtain, Mustafa Ghulam, Arshad Muhammad, Rajoka Muhammad Ibrahim
Department of Biochemistry, University of Agriculture, Faisalabad 38040, Pakistan; University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.
Department of Biochemistry, University of Agriculture, Faisalabad 38040, Pakistan; Department of Bioinformatics and Biotechnology, Government College University, Faisalabad 38000, Pakistan.
Biomed Res Int. 2017;2017:6232793. doi: 10.1155/2017/6232793. Epub 2017 Mar 6.
Industrially important enzymes and microbial biomass proteins have been produced from fungi for more than 50 years. High levels of crude protein as much as 45% are present in fungal biomass with balanced essential amino acids. The aim of this study was to access the potential of to produce fungal biomass protein from rice polishings. Maximum biomass yield was obtained at 5% (w/v) rice polishings after 72 h of incubation at 28°C at pH 4. Carbon and nitrogen ratio of 20 : 1 gave significantly higher production of fungal biomass protein. The FBP in the 75 L fermenter contained 49.50% crude protein, 32.00% true protein, 19.45% crude fiber, 9.62% ash, 11.5% cellulose content, and 0.325% RNA content. The profile of amino acids of final FBP exhibited that all essential amino acids were present in great quantities. The FBP produced by this fungus has been shown to be of good nutritional value for supplementation to poultry. The results presented in this study have practical implications in that the fungus could be used successfully to produce fungal biomass protein using rice polishings.
50多年来，人们一直在利用真菌生产具有工业重要性的酶和微生物生物质蛋白。真菌生物质中存在高达45%的粗蛋白，且必需氨基酸含量均衡。本研究的目的是评估利用米糠生产真菌生物质蛋白的潜力。在28°C、pH值为4的条件下培养72小时后，米糠浓度为5%（w/v）时可获得最大生物量产量。碳氮比为20∶1时，真菌生物质蛋白的产量显著更高。75升发酵罐中的真菌生物质蛋白含有49.50%的粗蛋白、32.00%的真蛋白、19.45%的粗纤维、9.62%的灰分、11.5%的纤维素含量和0.325%的RNA含量。最终真菌生物质蛋白的氨基酸谱显示，所有必需氨基酸含量都很高。已证明这种真菌产生的真菌生物质蛋白对家禽补充营养具有良好的营养价值。本研究结果具有实际意义，即该真菌可成功用于利用米糠生产真菌生物质蛋白。
