Verdam Maria Christina Dos Santos, Guilhon-Simplicio Fernanda, de Andrade Kleyton Cardoso, Fernandes Karina Lorena Meira, Machado Tallita Marques, da Silva Felipe Moura Araújo, de Souza Mayane Pereira, Koolen Hector Henrique Ferreira, Paula Cristiane da Silva, Hirota Beatriz Cristina Konopatzki, de Oliveira Vinícius Bednarczuk, Miyazaki Cristina Mayumi Sasaki, Kalegari Milena, Miguel Marilis Dallarmi, Stuelp-Campelo Patricia Maria, Miguel Obdulio Gomes
Department of Pharmacy, Federal University of Paraná, Curitiba, PR, Brazil; Faculty of Pharmacy, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Faculty of Pharmaceutical Sciences, Federal University of Amazonas, Manaus, AM, Brazil.
ScientificWorldJournal. 2017;2017:8367042. doi: 10.1155/2017/8367042. Epub 2017 Mar 6.
. is a promising neotropical genus, rich in flavonoids and triterpenes, with several proven pharmacological properties. Nevertheless, W. R. Anderson is an Amazonian species almost not studied. . To assess the antioxidant, anti-inflammatory, and analgesic activities of leaves. . We analyzed an ethanol extract and its fractions for polyphenol content and UHPLC-MS/MS, phosphomolybdenum, DPPH, TBARS antioxidant tests, formalin-induced pain, carrageenan-induced peritonitis, acetic acid-induced abdominal writhings, and hot plate assays. . All the samples showed high polyphenol content and antioxidant capacity in the phosphomolybdenum, DPPH, and TBARS tests. We identified ethyl gallate, quinic acid, gallic acid, catechin, epicatechin, quercetrin, and quercetin in the samples. was able to reduce leukocyte migration in the carrageenan-induced peritonitis by 43% and the licking time in the formalin test by 57%. In the acetic acid-induced writhing test, the chloroform (FCL) and ethyl acetate (FEA) fractions were the most active samples. FEA was selected for the hot plate test, where all the dosages tested (5, 50, and 200 mg·kg) showed significant analgesic activity. . has interesting analgesic and antioxidant activities, due to its high phenolic content, especially phenolic acids.
. 是一个很有前景的新热带属，富含黄酮类化合物和三萜类化合物，具有多种已证实的药理特性。然而，W. R. Anderson是一种几乎未被研究过的亚马逊物种。. 为了评估该植物叶子的抗氧化、抗炎和镇痛活性。. 我们分析了乙醇提取物及其馏分的多酚含量，并进行了超高效液相色谱-串联质谱（UHPLC-MS/MS）、磷钼酸、二苯基苦味酰基自由基（DPPH）、硫代巴比妥酸反应物（TBARS）抗氧化测试、福尔马林诱导的疼痛测试、角叉菜胶诱导的腹膜炎测试、乙酸诱导的扭体试验以及热板试验。. 在磷钼酸、DPPH和TBARS测试中，所有样品均显示出高多酚含量和抗氧化能力。我们在样品中鉴定出了没食子酸乙酯、奎宁酸、没食子酸、儿茶素、表儿茶素、槲皮苷和槲皮素。该植物能够使角叉菜胶诱导的腹膜炎中的白细胞迁移减少43%，并使福尔马林试验中的舔舐时间减少57%。在乙酸诱导的扭体试验中，氯仿（FCL）和乙酸乙酯（FEA）馏分是活性最高的样品。选择FEA进行热板试验，所有测试剂量（5、50和200 mg·kg）均显示出显著的镇痛活性。. 由于其高酚含量，尤其是酚酸，该植物具有有趣的镇痛和抗氧化活性。
