Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, 350002, P. R. China.
Soft Matter. 2017 Apr 19;13(16):3003-3012. doi: 10.1039/c7sm00350a.
Multiresponsive and biocompatible self-healing ε-PL/A-Pul/BPEI hydrogels were prepared in aqueous solution by Schiff base reaction with aldehyded pullulan (A-Pul), ε-poly-l-lysine (ε-PL) and branched polyethyleneimine (BPEI) as materials. The imine bonds were rapidly cross-linked into a hydrogel network within 80 s. Scanning electron microscopy images showed that the hydrogels exhibited a cross-linked structure with the average pore size from 58 to 82 μm. Rheology tests indicated that the hydrogels maintained good mechanical properties. Water contact angles and swelling studies suggested that the hydrogels could swell in water, with a max swell ratio of 1559%, and pH and temperature had an influence on the equilibrium swelling ratio. The hydrogels could be injected either before or after gelation, and they displayed a self-healing process in ddHO at room temperature based on the dynamic uncoupling and recoupling of the imine bonds. The MTT assays implied that the hydrogels were non-cytotoxic on mice bone marrow mesenchymal stem cells. Therefore, the hydrogels showed potential application in biomedical fields, and consequently further work was performed using the self-healing hydrogels as drug carriers in in vitro/vivo antitumor studies.
多响应性和生物相容性的自修复 ε-PL/A-Pul/BPEI 水凝胶是通过席夫碱反应在水溶液中制备的，所用材料为醛化普鲁兰（A-Pul）、ε-聚-L-赖氨酸（ε-PL）和支化聚乙烯亚胺（BPEI）。亚胺键在 80 秒内迅速交联成水凝胶网络。扫描电子显微镜图像显示，水凝胶呈现交联结构，平均孔径为 58-82μm。流变学测试表明，水凝胶保持良好的机械性能。水接触角和溶胀研究表明，水凝胶在水中可以溶胀，最大溶胀比为 1559%，并且 pH 值和温度对平衡溶胀比有影响。水凝胶可以在凝胶化之前或之后注入，并且它们基于亚胺键的动态解耦和再耦在室温下显示出自修复过程。MTT 测定表明水凝胶对小鼠骨髓间充质干细胞无细胞毒性。因此，水凝胶在生物医学领域有潜在的应用，因此进一步的工作是将自修复水凝胶用作体外/体内抗肿瘤研究中的药物载体。
