Eversole L R, Laipis P J
Department of Oral Diagnostic Sciences, College of Dentistry, University of Florida, Gainesville.
Oral Surg Oral Med Oral Pathol. 1988 May;65(5):545-50. doi: 10.1016/0030-4220(88)90137-5.
Oral squamous papillomas were segregated from other papillary lesions on the basis of histopathologic features. Twenty representative papillomas were evaluated for the presence of papillomavirus genus-specific antigen with the use of an immunoperoxidase technique. These same tumors were analyzed for human papillomavirus (HPV) types 2, 4, 6, and 11 with biotinylated full-length double-stranded DNA probes by in situ hybridization. Only one case exhibited papillomavirus antigen reactivity. Alternatively, seven of twenty cases (35%) yielded positive results for HPV 6 or 11 DNA; one papilloma exhibited a dual infection with both HPV 2 and 6 when assayed under conditions of high-stringency hybridization. It is concluded that some oral squamous papillomas harbor HPV genotypes akin to those encountered in genital tract condylomas. Viral DNA can be detected in the absence of capsid antigen immunoreactivity, thereby obviating the use of antigen detection assays for determining the presence or absence of virus.
口腔鳞状乳头状瘤根据组织病理学特征与其他乳头状病变区分开来。使用免疫过氧化物酶技术对20个代表性乳头状瘤进行乳头瘤病毒属特异性抗原检测。通过原位杂交，用生物素化的全长双链DNA探针分析这些相同的肿瘤中2、4、6和11型人乳头瘤病毒（HPV）。仅1例显示乳头瘤病毒抗原反应性。另外，20例中有7例（35%）HPV 6或11 DNA检测呈阳性；在高严格度杂交条件下检测时，1个乳头状瘤显示同时感染HPV 2和6。得出结论：一些口腔鳞状乳头状瘤携带的HPV基因型与生殖道尖锐湿疣中所见相似。在无衣壳抗原免疫反应性的情况下可检测到病毒DNA，因此无需使用抗原检测试验来确定病毒的有无。
