Okada Ayako, Nomura Yoshiaki, Sogabe Kaoru, Oku Hirofumi, Sato Gillbreath Arika, Hino Fumihiko, Hayashi Hideo, Yoshino Hirokazu, Utsunomiya Hisanori, Suzuki Kazuyuki, Koresawa Keizo, Koba Kenji, Uetani Kimiyuki, Kotoh Mami, Nishitsuji Naoyuki, Akutsu Satoshi, Nakasone Takakazu, Tobi Yasushi, Fukuzawa Yoichi, Yabuki Yoshihide, Naono Yoshinobu, Yajima Masataka, Shimizu Keita, Hanada Nobuhiro
Department of Translational Research, School of Dental Medicine, Tsurumi University.
J Oral Sci. 2017;59(1):63-69. doi: 10.2334/josnusd.16-0204.
Salivary hemoglobin (Hb) for screening of periodontitis is approved under the pharmaceutical affairs law of Japan. Two reagents are commercially available for the modified fecal occult blood test: Saliva Hemo Plus and OC-AUTO S Latex Reagent. We simultaneously measured split specimens from 561 samples by using these two methods and compared the differences and agreement between both methods. Moreover, saliva samples were collected from 10 subjects at five time points during the day for analysis of circadian variations and fluctuation. The Pearson's correlation coefficient for these two reagents was 0.794. The Bland-Altman plot of differences in salivary Hb levels measured by the two reagents indicated that the difference included fixed errors (0.55 μg/mL). On analysis of circadian variations, no statistically significant differences were observed using the Friedman test. However, fixed errors were observed between wake-up time and before dinner and before lunch and before dinner, and no random errors were observed by Bland-Altman analysis. In conclusion, the salivary Hb levels measured using OC-AUTO S Latex Reagent were lower than those measured using Saliva Hemo Plus, along with a tendency for higher levels in the morning. Thus, when performing salivary tests these observations must be considered.
用于牙周炎筛查的唾液血红蛋白（Hb）已根据日本药事法获得批准。有两种试剂可用于改良粪便潜血试验：唾液血红蛋白增强检测试剂（Saliva Hemo Plus）和OC - AUTO S乳胶试剂。我们使用这两种方法同时对561份样本的分离标本进行了检测，并比较了两种方法之间的差异和一致性。此外，在一天中的五个时间点从10名受试者收集唾液样本，以分析昼夜变化和波动情况。这两种试剂的皮尔逊相关系数为0.794。两种试剂测量的唾液Hb水平差异的布兰德 - 奥特曼图表明，差异包括固定误差（0.55μg/mL）。在昼夜变化分析中，使用弗里德曼检验未观察到统计学上的显著差异。然而，在起床时间与晚餐前以及午餐前与晚餐前之间观察到固定误差，并且通过布兰德 - 奥特曼分析未观察到随机误差。总之，使用OC - AUTO S乳胶试剂测量的唾液Hb水平低于使用唾液血红蛋白增强检测试剂测量的水平，并且上午有水平较高的趋势。因此，在进行唾液检测时必须考虑这些观察结果。
