Pine D S, Bourekas E C, Potter S S
Children's Hospital Research Foundation, Cincinnati, OH 45229.
Nucleic Acids Res. 1988 Apr 25;16(8):3359-73. doi: 10.1093/nar/16.8.3359.
The mys family of retrotransposons exhibits an interesting phylogenetic distribution with 500-1000 copies per haploid genome in the white-footed mouse Peromyscus leucopus and no copies detectable in the house mouse Mus musculus, even though most other repeated sequences are shared by these two species. Comparison of the DNA sequences from the 3' ends of five mys elements show that insertion occurs just upstream of a well-conserved 11 bp target sequence. Transcription patterns of the elements in brain, liver, heart, kidneys and gonads in Peromyscus leucopus are analyzed. Transcripts are found in all tissues examined, but they are remarkably heterogeneous in size. When four cloned elements are introduced into transgenic Mus musculus, however, discrete patterns of expression are revealed. Furthermore, a study of the structure of the concatemers of mys elements in the transgenic mice demonstrates that recircularization of injected linear molecules is an important event in concatemer generation.
逆转录转座子的mys家族呈现出有趣的系统发育分布，在白足鼠（Peromyscus leucopus）的单倍体基因组中，每个基因组有500 - 1000个拷贝，而在小家鼠（Mus musculus）中未检测到拷贝，尽管这两个物种共享大多数其他重复序列。对五个mys元件3'端的DNA序列进行比较表明，插入发生在一个高度保守的11 bp靶序列的上游。分析了白足鼠大脑、肝脏、心脏、肾脏和性腺中这些元件的转录模式。在所检查的所有组织中都发现了转录本，但它们的大小差异很大。然而，当将四个克隆元件导入转基因小家鼠时，揭示了离散的表达模式。此外，对转基因小鼠中mys元件串联体结构的研究表明，注射的线性分子的再环化是串联体产生中的一个重要事件。
