Silva Lisboa Dianny, Santos Cledir, Barbosa Renan N, Magalhães Oliane, Paiva Laura M, Moreira Keila A, Lima Nelson, Souza-Motta Cristina M
Department of Mycology, Centre of Biological Sciences, Federal University of Pernambuco, Recife, PE 50740-600, Brazil.
Department of Chemical Sciences and Natural Resources, Centro de Excelencia en Investigación Biotecnológica Aplicada al Medio Ambiente (CIBAMA), Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, Temuco 4811-230, Chile.
Int J Environ Res Public Health. 2017 Apr 1;14(4):373. doi: 10.3390/ijerph14040373.
Water contamination with large amounts of industrial textile coloured effluents is an environmental concern. For the treatment of textile effluents, white-rot fungi have received extensive attention due to their powerful capability to produce oxidative (e.g., ligninolytic) enzymes. In addition, other groups of fungi, such as species of and , have also been used for textile effluents treatment. The main aim of the present study was to requalify a Brazilian culture collection of 51 strains, isolated from different sources in Brazil and preserved in the oldest Latin-American Fungal Service Culture Collection, The Micoteca URM WDCM 804 (Recife, Brazil). Fungal isolates were re-identified through a polyphasic approach including macro- and micro-morphology and molecular biology, and screened for their capability to decolourise real effluents collected directly from storage tanks of a textile manufacture. URM 4950 presented the best performance on the dye decolourisation in real textile effluent and can be considered in a scale-up process at industrial level. Overall, the potential of strains in decolourising real textile dye present in textile effluent and the production of the oxidative enzymes Lac, LiP and MnP was demonstrated. Fungal strains are available in the collection e-catalogue to be further explored from the biotechnological point of view.
大量工业纺织染色废水造成的水污染是一个环境问题。对于纺织废水的处理，白腐真菌因其强大的氧化酶（如木质素分解酶）产生能力而受到广泛关注。此外，其他真菌类群，如曲霉属和青霉属的一些物种，也已被用于纺织废水处理。本研究的主要目的是重新鉴定保存在拉丁美洲最古老的真菌保藏中心——巴西累西腓的米科泰卡URM WDCM 804（The Micoteca URM WDCM 804）中的51株巴西曲霉属菌株，这些菌株是从巴西不同来源分离得到的。通过包括宏观和微观形态学以及分子生物学在内的多相方法对真菌分离株进行重新鉴定，并筛选它们对直接从一家纺织厂储存罐中收集的实际废水进行脱色的能力。URM 4950在实际纺织废水中对染料脱色表现出最佳性能，可考虑在工业规模上进行扩大培养。总体而言，证明了曲霉属菌株对纺织废水中实际纺织染料脱色以及产生氧化酶漆酶、木质素过氧化物酶和锰过氧化物酶的潜力。真菌菌株可在保藏中心的电子目录中获取，以便从生物技术角度进一步探索。
