Zoller L C, Carr K
Department of Anatomy, Boston University School of Medicine, Housman Research Center, Mass.
Acta Anat (Basel). 1988;131(4):265-70. doi: 10.1159/000146527.
Using morphometric techniques, we analyzed the effects of delta 9-tetrahydrocannabinol (THC) on the ultrastructure of rat preovulatory follicles. Rats were injected intraperitonally once daily with vehicle alone, or with vehicle plus THC on diestrus (D), metestrus (M) and diestrus (D), or on estrus (E), M and D. All animals were killed on proestrus. Nuclear, mitochondrial, lipid droplet and lysosomal volume density in the membrana granulosa were analyzed. There was no change in nuclear volume, but there were significant THC-induced decreases in mitochondrial and lipid droplet volumes in the peripheral region of the granulosa. There were increases in dense-body volume in all regions of the granulosa from rats given THC either on D alone, or M and D. Dense-body volume returned to control levels in rats injected on E, M and D. It appears that THC can affect follicular ultrastructure and the effects change with the number of injections.
我们运用形态测量技术，分析了δ9-四氢大麻酚（THC）对大鼠排卵前卵泡超微结构的影响。大鼠在动情间期（D）、动情后期（M）和动情间期（D），或在发情期（E）、M和D时，每天腹腔注射一次溶剂，或溶剂加THC。所有动物在动情前期处死。分析颗粒膜中细胞核、线粒体、脂滴和溶酶体的体积密度。细胞核体积无变化，但THC导致颗粒外周区域线粒体和脂滴体积显著减小。单独在D期给予THC，或在M期和D期给予THC的大鼠，颗粒所有区域的致密小体体积增加。在E期、M期和D期注射的大鼠，致密小体体积恢复到对照水平。看来THC可影响卵泡超微结构，且其影响随注射次数而变化。
