一种串联酶促sp-C-甲基化过程：将原位S-腺苷-L-甲硫氨酸形成与甲基转移相偶联。
A Tandem Enzymatic sp -C-Methylation Process: Coupling in Situ S-Adenosyl-l-Methionine Formation with Methyl Transfer.
作者信息
Sadler Joanna C, Humphreys Luke D, Snajdrova Radka, Burley Glenn A
机构信息
GlaxoSmithKline Medicines Research Centre, Gunnels Wood Road, Stevenage, SG1 2NY, UK.
WestCHEM, Department of Pure and Applied Chemistry, University of Strathclyde, 295 Cathedral Street, Glasgow, G1 1XL, UK.
出版信息
Chembiochem. 2017 Jun 1;18(11):992-995. doi: 10.1002/cbic.201700115. Epub 2017 Apr 27.
A one-pot, two-step biocatalytic platform for the regiospecfic C-methylation and C-ethylation of aromatic substrates is described. The tandem process utilises SalL (Salinospora tropica) for in situ synthesis of S-adenosyl-l-methionine (SAM), followed by alkylation of aromatic substrates by the C-methyltransferase NovO (Streptomyces spheroides). The application of this methodology is demonstrated for the regiospecific labelling of aromatic substrates by the transfer of methyl, ethyl and isotopically labelled CH CD and CD groups from their corresponding SAM analogues formed in situ.
本文描述了一种用于芳香族底物区域特异性C-甲基化和C-乙基化的一锅两步生物催化平台。该串联过程利用热带盐单胞菌（Salinospora tropica）的SalL原位合成S-腺苷-L-甲硫氨酸（SAM），随后通过球形链霉菌（Streptomyces spheroides）的C-甲基转移酶NovO对芳香族底物进行烷基化。通过原位形成的相应SAM类似物转移甲基、乙基和同位素标记的CH CD及CD基团，证明了该方法在芳香族底物区域特异性标记中的应用。
Zotero 插件