Shiozawa K, Hayashi M, Akiyama M, Nishina T, Nakatsugawa S, Fukushima H, Asakawa Y
Shizuoka Prefectural Institute of Public Health and Environmental Science, Japan.
Appl Environ Microbiol. 1988 Mar;54(3):818-21. doi: 10.1128/aem.54.3.818-821.1988.
Yersinia pseudotuberculosis was isolated from retail pork and from healthy swine throats. These wild-type strains and their representative cured isogenic strains were tested for the presence of plasmids and several virulence factors, and these characteristics were compared with those of virulent strains from humans. Two pork isolates (serotype IVB) and four swine isolates (serotypes IIB, IIC, III, and IVB) harbored a 42- to 48-megadalton plasmid which had similar fragmentation patterns resulting from digestion with restriction endonuclease. These six strains were lethal for mice via oral challenge and were positive in autoagglutination and calcium dependency tests. They also invaded HeLa cells and induced cytotoxicity. Histopathological examination and indirect fluorescent-antibody staining provided definite evidence of the pathogenicity of these strains when tissue sections from orally infected mice were used. The virulence factors of wild-type pork and swine isolates with the 42- to 48-megadalton plasmid were identical to those of two human isolates (serotypes IVB and VB). Hence, these pork and swine isolates should be considered potentially pathogenic for humans. The finding suggests that retail pork and swine may play an important role in the epidemiology of human infections caused by Y. pseudotuberculosis.
从零售猪肉和健康猪的咽喉中分离出了假结核耶尔森菌。对这些野生型菌株及其代表性的治愈同基因菌株进行了质粒和几种毒力因子的检测，并将这些特征与来自人类的强毒株的特征进行了比较。两株猪肉分离株（血清型IVB）和四株猪分离株（血清型IIB、IIC、III和IVB）携带一个42至48兆道尔顿的质粒，该质粒经限制性内切酶消化后具有相似的片段化模式。这六株菌株经口服攻击对小鼠具有致死性，并且在自凝和钙依赖性试验中呈阳性。它们还侵袭HeLa细胞并诱导细胞毒性。当使用经口服感染小鼠的组织切片时，组织病理学检查和间接荧光抗体染色为这些菌株的致病性提供了确切证据。携带42至48兆道尔顿质粒的野生型猪肉和猪分离株的毒力因子与两株人类分离株（血清型IVB和VB）的毒力因子相同。因此，这些猪肉和猪分离株应被视为对人类具有潜在致病性。这一发现表明，零售猪肉和猪可能在假结核耶尔森菌引起的人类感染流行病学中发挥重要作用。
