Ricard B, Fourquet P, Massacrier A, Couraud F
Laboratoire de Biochimie et de Biologie Cellulaire, INSERM U 172, CNRS UA 1179, Faculté de Médecine Nord, Marseille, France.
Biochem Biophys Res Commun. 1988 May 16;152(3):1031-7. doi: 10.1016/s0006-291x(88)80387-5.
A monoiodo derivative of rat atrial natriuretic factor (rANF) was shown to specifically bind to rat brain neurones in culture with low binding site capacity (10-20 fmoles per mg of protein) and high affinity (Kd = 50-100 pM). Several analogs of both rat and human ANF competed with 125I-rANF. No change in the number of binding sites was detected upon morphological differentiation of neurones in vitro. Finally a photoreactive derivative of 125I-rANF was prepared and photoaffinity labeling experiments carried out on cultured neurones. After reduction of disulfide bridges, a single band of Mr 60,000 was specifically labeled whereas without reduction, two labeled components of Mr 60,000 and 117,000 were detected.
大鼠心房利钠因子（rANF）的单碘衍生物显示出与培养的大鼠脑神经元特异性结合，其结合位点容量低（每毫克蛋白质10 - 20飞摩尔）且亲和力高（解离常数Kd = 50 - 100皮摩尔）。大鼠和人类ANF的几种类似物与125I - rANF竞争。在体外神经元形态分化时未检测到结合位点数量的变化。最后制备了125I - rANF的光反应性衍生物，并在培养的神经元上进行了光亲和标记实验。在二硫键还原后，一条分子量为60,000的单带被特异性标记，而未还原时，检测到分子量为60,000和117,000的两个标记成分。
