Yip C C, Laing L P, Flynn T G
J Biol Chem. 1985 Jul 15;260(14):8229-32.
Synthetic rat atrial natriuretic factor (ANF) was derivatized with the N-hydroxysuccinimide ester of [125I]iodoazidosalicylic acid to yield a radioactive photoaffinity probe. Incubation of purified plasma membranes from rat kidney cortex with this photoaffinity probe resulted in the specific labeling of a 140-kDa glycoprotein. The photoaffinity labeling of this protein was inhibited by ANF but not by reduced and alkylated ANF nor by other unrelated peptides. A 140-kDa band was also specifically labeled in liver plasma membranes but not in adipocyte plasma membranes. These observations suggest strongly that the 140-kDa glycoprotein is the ANF receptor.
合成的大鼠心房利钠因子（ANF）用[125I]碘叠氮水杨酸的N-羟基琥珀酰亚胺酯进行衍生化，以产生一种放射性光亲和探针。用这种光亲和探针孵育大鼠肾皮质的纯化质膜，导致一种140 kDa糖蛋白的特异性标记。这种蛋白的光亲和标记被ANF抑制，但不被还原和烷基化的ANF或其他无关肽抑制。在肝质膜中也特异性标记出一条140 kDa的条带，但在脂肪细胞质膜中未标记出。这些观察结果强烈表明，140 kDa糖蛋白是ANF受体。
