BIOSYST-MeBioS, KU Leuven, W. de Croylaan 42, 3001 Leuven, Belgium.
Flanders Centre of Postharvest Technology, De Croylaan 42, 3001 Heverlee, Belgium.
Food Chem. 2017 Aug 15;229:296-303. doi: 10.1016/j.foodchem.2017.02.086. Epub 2017 Feb 20.
Belgian endive (Cichorium intybus L. var. foliosum Hegi), a popular produce in northern Europe, has been thoroughly studied in regard to its bitter sesquiterpene lactones content. Much less is known about on its sweetness and crunchiness, which are typically linked to the content of polar compounds such as sugars, organic acids and salts. Through HILIC-HPLC-MS, it was shown that simple sugars, amino acids, and potassium chloride are abundant in Belgian endive extracts. Subsequently, a HILIC-HPLC-ELSD method for the analysis of such compounds with run times below six minutes was developed. Recoveries varied between 80 and 110% and an average reproducibility was 7.5RSD%. Finally, the method was applied to the study of three difference Belgian endive varieties. Takine, a variety known for its sweet taste, was found to contain significantly higher levels of fructose, and lower levels of potassium and glutamine.
比利时菊苣(菊苣 Cichorium intybus L. var. foliosum Hegi),一种在北欧广受欢迎的农产品,其苦倍半萜内酯的含量已得到深入研究。然而,人们对其甜味和爽脆口感的了解相对较少,这些口感通常与极性化合物如糖、有机酸和盐的含量有关。通过 HILIC-HPLC-MS 分析表明,比利时菊苣提取物中含有丰富的单糖、氨基酸和氯化钾。随后,开发了一种 HILIC-HPLC-ELSD 分析方法,该方法的运行时间在六分钟以下。回收率在 80%至 110%之间变化,平均重现性为 7.5%RSD。最后,该方法应用于三种不同比利时菊苣品种的研究。Takine 是一种以甜味著称的品种,其果糖含量明显较高,钾和谷氨酰胺含量较低。