Lee Ga-Young, Kim Eiseul, Yang Seung-Min, Kim Hae-Yeong
Institute of Life Sciences & Resources, Department of Food Science and Biotechnology, Kyung Hee University, Yongin 17104, Korea.
Foods. 2022 Aug 14;11(16):2449. doi: 10.3390/foods11162449.
Granular ark (), broughton's ribbed ark (), and half-crenate ark () are important fishery resources throughout Asia; granular ark exhibiting a higher economic value due to its rarity. However, due to the similar morphological characteristics of the three species, the less valuable species could be exploited for food fraud. In this study, we developed a rapid on-site identification method based on a microfluidic chip for the detection of the three ark shell species. We designed new species-specific primers, targeting the genes encoding mitochondrial cytochrome b or cytochrome c oxidase I, for the identification of the three ark shells and estimated their specificity against 17 species, which amplified only the target species. The sensitivity of each primer was 0.001 ng. In addition, this method was further improved to develop a direct ultrafast polymerase chain reaction (PCR) for on-site food monitoring, which would allow for completing the entire procedure (from sampling to obtaining the results) within 25 min without DNA extraction. Our direct, ultrafast PCR was successfully applied to differentiate the three species from 29 commercial products. Therefore, this assay could be used as a rapid and cost-effective approach for the on-site identification of ark shells in commercial food products.
粒突箱形螺、布氏细纹箱形螺和半锯齿箱形螺是亚洲重要的渔业资源；粒突箱形螺因其稀有性而具有较高的经济价值。然而，由于这三种物种形态特征相似，价值较低的物种可能会被用于食品欺诈。在本研究中，我们开发了一种基于微流控芯片的快速现场鉴定方法，用于检测这三种箱形螺物种。我们设计了新的物种特异性引物，靶向编码线粒体细胞色素b或细胞色素c氧化酶I的基因，用于鉴定这三种箱形螺，并评估了它们对17个物种的特异性，这些引物仅扩增目标物种。每个引物的灵敏度为0.001 ng。此外，该方法进一步改进，开发了一种用于现场食品监测的直接超快聚合酶链反应（PCR），无需DNA提取即可在25分钟内完成整个过程（从采样到获得结果）。我们的直接超快PCR成功应用于区分29种商业产品中的这三种物种。因此，该检测方法可作为一种快速且经济高效的方法，用于现场鉴定商业食品中的箱形螺。
