College of Biological and Pharmaceutical Engineering, Jilin Agricultural Science and Technology University, Jilin 132101, Jilin, China.
College of Agronomy, Jilin Agricultural Science and Technology University, Jilin 132101, Jilin, China.
Food Chem. 2021 May 30;345:128840. doi: 10.1016/j.foodchem.2020.128840. Epub 2020 Dec 11.
A simple, rapid and accurate detection method for the authentication of animal species is urgently required in the food detection field. The present study established a horse-specific polymerase chain reaction integrated with a lateral flow sensor assay (Horse-PCR-LFS) for the rapid detection of horse meat. In this test, a cytb gene sequence of horse was amplified using PCR, the PCR amplicon was checked with the lateral flow sensor assay, and the result of the sensor can be read within 2-3 min by the naked eye. The detection limit of the test was up to 0.01% horse meat in artificially adulterated meat mixtures, the assay also successfully detected horse DNA in various commercial food samples. As a rapid and user-friendly molecular detection tool, this test provides an accurate detection format for the identification of horse and offers solutions to problems related to animal meat adulteration and animal-origin food safety and traceability.
在食品检测领域，人们迫切需要一种简单、快速、准确的动物物种鉴定方法。本研究建立了一种马特异性聚合酶链反应与侧向流传感器检测相结合的方法（Horse-PCR-LFS），用于快速检测马肉。在该试验中，使用 PCR 扩增马的 cytb 基因序列，用侧向流传感器检测 PCR 扩增产物，通过肉眼在 2-3 分钟内即可读取传感器的检测结果。该检测方法的检测限可达到人工掺杂肉混合物中 0.01%的马肉，该方法还成功地检测了各种商业食品样品中的马 DNA。作为一种快速友好的分子检测工具，该试验为马的鉴定提供了一种准确的检测方式，为解决动物肉掺假以及动物源性食品安全和可追溯性问题提供了方案。
