College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, Nanjing 210023, China.
College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, Nanjing 210023, China.
Food Chem. 2017 Aug 15;229:347-353. doi: 10.1016/j.foodchem.2017.02.094. Epub 2017 Feb 21.
This article developed a method to detect two antioxidants (butylated hydroxyl anisole (BHA), butylated hydroxyl toluene (BHT)) and twelve pesticides (dichlorvos, pirimicarb, prothiofos, fenitrothion, ethoprophos, malathion, beta-Cypermethrin, profenofos, diazinon, propoxur, procymidon, captan) in blended oil samples after their extraction simultaneously. The establishment of the method was based on two-step process of screening and optimization experiment design. With a Plackett-Burman (PB) design, significant parameters were found by screening experiment and single factor experiment accompanied by a central composite design (CCD) experiment were used to make important parameters optimal. The coefficients of determination (r) was between 0.9931 and 0.9996 while the limits of quantification (LOQs) and limits of detection (LODs) were found in scope of 0.002-0.04mg·kg and 0.0006-0.0012mg·kg. Recovery values of analytes were above 74%, at the same time the relative standard deviations (RSDs) under 10% at the concentrations ranging from 0.05mg·kg to 0.20mg·kg. To sum up, this method with shorter time and fewer consumption of reagents could be employed in various occasions to detect antioxidants and pesticides.
本文建立了一种同时检测混合油样中两种抗氧化剂（丁基羟基茴香醚（BHA）、丁基羟基甲苯（BHT））和十二种农药（敌百虫、灭多威、丙溴磷、三唑磷、乙硫磷、马拉硫磷、β-氯氰菊酯、丙溴磷、敌敌畏、残杀威、仲丁威、克百威）的方法。该方法基于两步筛选和优化实验设计。通过筛选实验和单因素实验，确定了显著参数，然后采用中心复合设计（CCD）实验对重要参数进行优化。该方法的决定系数（r）在 0.9931 到 0.9996 之间，定量限（LOQ）和检测限（LOD）在 0.002-0.04mg·kg 和 0.0006-0.0012mg·kg 范围内。在 0.05mg·kg 至 0.20mg·kg 的浓度范围内，分析物的回收率均在 74%以上，同时相对标准偏差（RSD）低于 10%。总的来说，该方法具有时间短、试剂消耗少的优点，可用于各种场合检测抗氧化剂和农药。
