Dai Yingping, Wang Ting, Hu Xiaoya, Liu Shuzhao, Zhang Ming, Wang Chengyin
College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, China; Jiangsu Key Laboratory of Environmental Engineering and Monitoring, Yangzhou 225002, China.
College of Environmental Science & Engineering, Yangzhou University, 196 Hua-Yang-Xi Road, Yangzhou 225002, China.
Food Chem. 2017 Aug 15;229:432-438. doi: 10.1016/j.foodchem.2017.02.093. Epub 2017 Feb 21.
Microcantilever-based immunosensor is a next-generation electromechanical technique with broad application in biological detection. In this paper, we reported a microcantilever-based immunosensor that quantitatively detect the carbofuran, by using monoclonal antibodies to carbofuran as the receptor molecules. The surface of gold-coated microcantilever was chemically modified by the crosslinking of l-cysteine (l-cys)/glutaraldehyde (GA). The monoclonal antibodies to carbofuran were then immobilized on the side of the microcantilever to fabricate the immunosensor, the mechanical bending induced by antigen-antibody specific binding under an experimental environment. Under the optimized conditions, immunosensor detected carbofuran showed a good linear relationship over the range from 1.0×10 to 1.0×10g/L (R=0.998), with a detection limit of 0.1ng/mL. Moreover, the proposed immunosensor exhibited high sensitivity, specificity and good stability and can be successfully applied in the carbofuran determination in soil and vegetable samples with satisfactory results.
基于微悬臂梁的免疫传感器是一种在生物检测中具有广泛应用的下一代机电技术。在本文中，我们报道了一种基于微悬臂梁的免疫传感器，该传感器通过使用对克百威的单克隆抗体作为受体分子来定量检测克百威。通过l-半胱氨酸（l-cys）/戊二醛（GA）交联对金涂层微悬臂梁表面进行化学修饰。然后将对克百威的单克隆抗体固定在微悬臂梁的侧面以制造免疫传感器，在实验环境下抗原-抗体特异性结合会引起机械弯曲。在优化条件下，检测克百威的免疫传感器在1.0×10至1.0×10g/L范围内显示出良好的线性关系（R = 0.998），检测限为0.1ng/mL。此外，所提出的免疫传感器具有高灵敏度、特异性和良好的稳定性，并且可以成功应用于土壤和蔬菜样品中克百威的测定，结果令人满意。
