基于波导的荧光免疫传感器同时检测克百威和 3-羟基克百威。
Waveguide-Based Fluorescent Immunosensor for the Simultaneous Detection of Carbofuran and 3-Hydroxy-Carbofuran.
机构信息
Key Laboratory of A & F Environmental Processes and Ecological Regulation of Hainan Province, College of Ecology and Environment, Hainan University, Haikou 570228, China.
State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, China.
出版信息
Biosensors (Basel). 2020 Nov 27;10(12):191. doi: 10.3390/bios10120191.
Carbofuran (CBF) is an efficient and broad-spectrum insecticide. As testing indicators for water quality and agricultural products, CBF and its metabolite 3-hydroxy-carbofuran (3-OH-CBF) are regulated by many countries. The detection of CBF and 3-OH-CBF is of great importance for the environment and human health. However, an immunosensor detection method for the simultaneous analysis of CBF and 3-OH-CBF remains unavailable. Herein, we report a waveguide-based fluorescent immunosensor for detecting CBF and 3-OH-CBF, synchronously. The immunosensor is based on a broad-spectrum monoclonal antibody with high binding affinity against CBF and 3-OH-CBF. The linear detection ranges for CBF and 3-OH-CBF are 0.29-2.69 and 0.12-4.59 μg/L, with limits of detection of 0.13 μg/L for CBF and 0.06 μg/L for 3-OH-CBF, respectively. The whole detection process for each cycle is approximately 30 min. The results show a good application prospect for the rapid detection of CBF and 3-OH-CBF in water or agricultural products.
呋喃丹（CBF）是一种高效、广谱的杀虫剂。作为水质和农产品的检测指标，CBF 及其代谢物 3-羟基呋喃丹（3-OH-CBF）受到许多国家的监管。因此，对 CBF 和 3-OH-CBF 的检测对于环境和人类健康具有重要意义。然而，目前仍然缺乏同时分析 CBF 和 3-OH-CBF 的免疫传感器检测方法。本文报道了一种基于波导的荧光免疫传感器，用于同步检测 CBF 和 3-OH-CBF。该免疫传感器基于一种广谱单克隆抗体，对 CBF 和 3-OH-CBF 具有高结合亲和力。CBF 和 3-OH-CBF 的线性检测范围分别为 0.29-2.69μg/L 和 0.12-4.59μg/L，检测限分别为 0.13μg/L 和 0.06μg/L。每个检测周期的整个检测过程大约需要 30 分钟。结果表明，该方法在快速检测水样和农产品中的 CBF 和 3-OH-CBF 方面具有良好的应用前景。
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