Chen C C, Lin-Shiau S Y
Pharmacological Institute, College of Medicine, National Taiwan University, Taipei.
Biochem Pharmacol. 1988 May 1;37(9):1661-6. doi: 10.1016/0006-2952(88)90424-8.
(Na+ -K+)ATPase activity in erythrocyte membranes of spontaneously (SHR), one kidney-one wrapped, and deoxycorticosterone acetate (DOCA)-NaCl hypertensive rats was studied. (Na+ -K+) ATPase activity decreased in both prehypertensive (6 weeks old) and hypertensive (14 weeks old) stages of SHR, suggesting that the alteration of this enzymic activity may be due to a pre-existing defect in the membrane rather than being a consequence of hypertension. By contrast, (Na+ -K+)ATPase activity remained unchanged in the one kidney-one wrapped hypertensive rats, whereas that of one kidney-one wrapped normotensive rats as well as that of DOCA-NaCl hypertensive rats was increased significantly (P less than 0.05). These changes were specific for (Na+ -K+) ATPase, since Mg2+-ATPase activity was not altered. The susceptibility of (Na+ -K+)ATPase to the inhibitory action of ouabain was not changed significantly. These findings indicate that (Na+ -K+)ATPase activities of erythrocyte membranes isolated from the different types of hypertensive rats were subject to different changes. Whether this phenomenon applies to the clinical distinctions among the various types of hypertension remains a subject for further investigation.
研究了自发性高血压大鼠（SHR）、单肾-单包被高血压大鼠以及醋酸脱氧皮质酮（DOCA）-氯化钠高血压大鼠红细胞膜中的（Na + -K +）ATP酶活性。在SHR的高血压前期（6周龄）和高血压期（14周龄），（Na + -K +）ATP酶活性均降低，这表明该酶活性的改变可能是由于膜中预先存在的缺陷，而非高血压的结果。相比之下，单肾-单包被高血压大鼠的（Na + -K +）ATP酶活性保持不变，而单肾-单包被正常血压大鼠以及DOCA-氯化钠高血压大鼠的该酶活性则显著增加（P小于0.05）。这些变化对（Na + -K +）ATP酶具有特异性，因为Mg2 + -ATP酶活性未改变。（Na + -K +）ATP酶对哇巴因抑制作用的敏感性没有显著变化。这些发现表明，从不同类型高血压大鼠分离的红细胞膜中的（Na + -K +）ATP酶活性受到不同的影响。这种现象是否适用于不同类型高血压的临床差异仍有待进一步研究。
