Bukholm G, Bjørnland K, Ellekjaer H, Berdal B P, Degré M
Kaptein W. Wilhelmsen og Frues Bakteriologiske Institutt, University of Oslo, Norway.
APMIS. 1988 May;96(5):400-6.
When mouse fibroblast L-929 cells were pre-infected with vesicular stomatitis virus, an enhancement of invasiveness by Salmonella typhimurium was observed. The effect was more pronounced when higher virus doses were used. Short-time (5 h) pre-incubation with virus caused a moderate enhancement of invasiveness. When virus pre-incubation time was increased to 8 h or 13 h, a further enhancement was observed. Results obtained after pre-incubation with UV inactivated virus were similar to that achieved by the short-time pre-incubation with the corresponding viable virus preparation. This indicates (i) an early phase of virus infection, when virus causes enhancement of invasiveness that is not dependent on viral nucleic acid induced metabolism, and (ii) a later phase, when virus-induced metabolism is necessary for the enhancement. When virus and bacteria were given concomitantly to infant mice, lethality was increased compared to groups that only received virus or bacteria. The data indicate that vesicular stomatitis virus aggravates infection with a facultatively intracellular bacterium, partly by enhancing the invasiveness of the bacteria.
当小鼠成纤维细胞L-929细胞预先感染水疱性口炎病毒时，观察到鼠伤寒沙门氏菌的侵袭力增强。使用更高病毒剂量时，这种效果更明显。与病毒进行短时间（5小时）预孵育会导致侵袭力适度增强。当病毒预孵育时间增加到8小时或13小时时，观察到进一步增强。用紫外线灭活病毒预孵育后获得的结果与用相应活病毒制剂进行短时间预孵育所达到的结果相似。这表明（i）病毒感染的早期阶段，此时病毒引起侵袭力增强，且不依赖于病毒核酸诱导的代谢，以及（ii）后期阶段，此时病毒诱导的代谢对于增强是必要的。当病毒和细菌同时给予幼鼠时，与仅接受病毒或细菌的组相比，致死率增加。数据表明水疱性口炎病毒部分通过增强细菌的侵袭力来加重兼性胞内细菌的感染。
