Day E P, Peterson J, Bonvoisin J J, Young L J, Wilkerson J O, Siegel L M
Gray Freshwater Biological Institute, University of Minnesota, Navarre 55392.
Biochemistry. 1988 Mar 22;27(6):2126-32. doi: 10.1021/bi00406a046.
The saturation magnetizations of the sulfite complex of oxidized sulfite reductase and the nitrite complex of oxidized nitrite reductase have been measured to determine their spin state. Each shows the saturation magnetization signal of a spin S = 1/2 state with sigma g2 = 16, which is typical of low-spin ferrihemes. However, the EPR spectra of these complexes lack the expected signal intensity of a spin S = 1/2 state. Indeed, one of these complexes is EPR silent. The reasons for this unexpectedly low EPR signal intensity are considered.
已测量氧化型亚硫酸盐还原酶的亚硫酸盐复合物和氧化型亚硝酸盐还原酶的亚硝酸盐复合物的饱和磁化强度，以确定它们的自旋态。每一种都显示出自旋S = 1/2态的饱和磁化强度信号，其中σg2 = 16，这是低自旋高铁血红素的典型特征。然而，这些复合物的电子顺磁共振光谱缺乏自旋S = 1/2态预期的信号强度。事实上，其中一种复合物是电子顺磁共振沉默的。本文考虑了这种意外的低电子顺磁共振信号强度的原因。
