Bundscherer Anika C, Malsy Manuela, Bitzinger Diane I, Wiese Christoph H R, Gruber Michael A, Graf Bernhard M
Department of Anesthesiology, University of Regensburg, Regensburg, Germany
Department of Anesthesiology, University of Regensburg, Regensburg, Germany.
Anticancer Res. 2017 Apr;37(4):1941-1945. doi: 10.21873/anticanres.11534.
Evidence is growing that the risk of cancer dissemination may be enhanced during the perioperative period. Whether particular anesthetic techniques influence oncological outcome is still under discussion. For pain management, lidocaine can be administered perioperatively by intravenous, intraperitoneal or epidural infusion. Here we investigated the effect of lidocaine on colon carcinoma cell lines (HT-29 and SW480) in vitro.
ELISA BrdU (Roche) for cell proliferation and FITC Annexin V detection kit (BD Pharming) for apoptosis analysis were applied. Cell-cycle profiles were investigated by flow cytometry.
Cell-cycle arrest was induced in both cell lines by 1000 μM lidocaine, while no inhibition of cell proliferation was detected. Apoptosis decreased in SW480 but not in HT-29 cells.
Lidocaine induces cell-cycle arrest in both colon carcinoma cell lines in vitro. The effective drug concentration can be obtained by local infiltration.
越来越多的证据表明，围手术期癌症播散的风险可能会增加。特定的麻醉技术是否会影响肿瘤学结局仍在讨论中。对于疼痛管理，利多卡因可在围手术期通过静脉、腹腔或硬膜外输注给药。在此，我们在体外研究了利多卡因对结肠癌细胞系（HT-29和SW480）的影响。
应用用于细胞增殖分析的ELISA BrdU（罗氏公司）和用于凋亡分析的FITC膜联蛋白V检测试剂盒（BD制药公司）。通过流式细胞术研究细胞周期分布。
1000μM利多卡因在两种细胞系中均诱导了细胞周期停滞，但未检测到对细胞增殖的抑制作用。SW480细胞中的凋亡减少，而HT-29细胞中未减少。
利多卡因在体外可诱导两种结肠癌细胞系的细胞周期停滞。通过局部浸润可获得有效的药物浓度。
