Department of Anesthesiology, University Hospital of Regensburg, Regensburg, Germany
Department of Anesthesiology, University Hospital of Regensburg, Regensburg, Germany.
Anticancer Res. 2019 Nov;39(11):6049-6055. doi: 10.21873/anticanres.13811.
BACKGROUND/AIM: For patients undergoing cancer surgery, the risk for cancer progression is enhanced during the perioperative period. To what extent the type of anesthetic can affect the metastatic process and finally the outcome of patients with cancer is under debate. For this reason, the aim of this study was to investigate the effects of the volatile anesthetics sevoflurane and desflurane on colon cancer cells in vitro.
SW480 colon carcinoma cells were exposed for 3 or 6 h to sevoflurane (1 or 2.5 vol%) or desflurane (6 or 12 vol%). Cell cycle distribution was analyzed by flow cytometry after a 24-72 h recovery and apoptosis was detected by annexin V staining after a 0-48 h recovery. Viability was tested by measuring ATP content after 0 and 24 h recovery.
Treatment with sevoflurane or desflurane caused no or only slight changes in cell-cycle distribution and apoptosis rate. Desflurane at 12vol% significantly reduced cell viability by 17±25% and 11±22% after 3 and 6 h incubation and 24 h recovery, respectively, while 2.5 vol% sevoflurane slightly increased viability.
At clinically relevant concentrations, sevoflurane and desflurane had only slight effects on SW480 colon cancer cells in vitro.
背景/目的：对于接受癌症手术的患者，围手术期癌症进展的风险增加。麻醉类型在多大程度上会影响转移过程，并最终影响癌症患者的预后，这仍存在争议。出于这个原因，本研究旨在研究挥发性麻醉剂七氟醚和地氟醚对体外结肠癌 SW480 细胞的影响。
SW480 结肠癌细胞分别暴露于七氟醚（1 或 2.5 体积%）或地氟醚（6 或 12 体积%）3 或 6 小时。24-72 小时恢复后，通过流式细胞术分析细胞周期分布，0-48 小时恢复后通过 Annexin V 染色检测细胞凋亡。0 和 24 小时恢复后，通过测量 ATP 含量来检测细胞活力。
七氟醚或地氟醚处理对细胞周期分布和细胞凋亡率没有或只有轻微影响。12 体积%地氟醚孵育 3 小时和 6 小时后，24 小时恢复时细胞活力分别显著降低 17±25%和 11±22%，而 2.5 体积%七氟醚略微增加了细胞活力。
在临床相关浓度下，七氟醚和地氟醚对体外 SW480 结肠癌细胞仅有轻微影响。
